猪鞭虫和斯氏鞭虫线粒体pnad4和pcox1基因的扩增与序列分析

Amplification and Sequences Analysis of Mitochondrial pnad4 and pcox1 Gene in Trichuris suis and Trichuris skrjabini

以采自广东不同地区的猪鞭虫(Trichuris suis)和斯氏鞭虫(Trichuris skrjabini)为研究对象,扩增线粒体基因组的pnad4和pcox1基因,将扩增产物纯化后克隆至pMD18-T载体,对阳性菌落进行测序和序列分析。结果表明,来源于不同地区的鞭虫上述两个基因种内相对保守,差异性不大,但种间差异较大。猪鞭虫和斯氏鞭虫的pnad4长度均为468 bp,相互间有162个种间遗传标记位点;猪鞭虫和斯氏鞭虫的pcox1长度分别为600 bp与438 bp,相互间有156个种间遗传标记位点。两种鞭虫之间的pnad4基因差异率为45.1%-45.9%,pcox1基因差异率为44.1%-45.4%。上述线粒体两个基因均可作为区分两种线虫的种间遗传标记。

The mitochondria NADH dehydrogenase subunit gene IV （ pnad4 ） and cytochrome coxidase subunit l （ pcoxl ） of Trichuris stds and Trichuris skrjabini that collected from different areas of Guangdong province were amplificated, cloned and sequenced. The results showed that the lengths of pnad4 genes sequences both Trichuris suis and Trichuris skrjabini were 468 bp. The lengths of Trichuris suis and Trichuris skrjabini peoxl genes sequences were 600 bp and 438 bp, respectively. There were 162 interspecies genetic marker sites of the two species of Trichuris in the fragment of pnad4, and 156 in the fragment of pcoxl based on the sequence analysis. The difference of the two species of Trichuris in the fragment of pnad4 gene was 45.1%-45.9%, and the difference in the fragment of pcoxl gene was 44.1%-45.4%. The two fragments of the mtDNA can be used as genetic markers to distinguish the Trichuris suis and Trichuris skrjabini.