摘要
目的:研究mdr1b/a基因siRNA(small interfering RNA)逆转膀胱癌的多药耐药性。方法:设计mdr1b/a基因的siRNA,转染膀胱癌T24/ADM细胞,用RT-PCR分析mRNA的表达,Rh123分析P-gp活性,流式细胞仪检测细胞内阿霉素积累量。结果:siRNA能明显地逆转T24/ADM细胞的多药耐药,使mdr1b/a基因的mRNA表达水平下调,细胞内阿霉素积累量显著增加(P<0.05)。结论:mdr1b/a基因siRNA能够逆转膀胱癌的多药耐药性。
Objective： To investigate the effect of mdrlb/mdrla siRNA on multidrug resistance of bladder carcinoma cell line T24/ADM. Methods： The proper mdrlb/a siRNA was designed, synthesized and transfected into bladder carcinoma cell line T24/ADM. Expression level of mdrlb/a mRNA was deter- mined by PCR. The activity of Pgp was measured by Rhodamine 123 efflux assay, The intracellular accumulation of ADM was assayed with flow cytometry. Results： After treated with siRNA, the expression level of mdrlb/ was increased si ante of bladder a mRNA in T24/ADM decreased obviously, and the intracellular accumulation of ADM gnificantly （P〈0.05）. Conclusions： The mdrlb/asiRNA can reverse the multidrug resist- carcinoma cell line T24/ADM.
出处
《海南医学院学报》
CAS
2012年第7期865-868,共4页
Journal of Hainan Medical University
基金
湖北省教育厅科学技术研究计划重点项目(D20082404)~~
