高效液相色谱法同时测定复方丹参片6种活性成分含量

Content Determination of Six Kinds of Active Ingredients in Compound DanShen Tablets with HPLC Simultaneously

目的:建立同时测定复方丹参片中源于丹参药材的6种活性成分含量的高效液相色谱方法。方法:采用VP-ODS C18柱进行分离(4.6 mm×150 mm,5μm,Shimadzu,Japan);流动相采用1%醋酸(A)和乙腈(B)二元梯度系统;采集波长:263 nm;柱温:40℃;流速:1.0 mL/min。结果:6种活性成分在所建浓度范围内线性关系良好r,2均大于0.999;方法精密度RSD小于4.12%;平均加样回收率均大于98.00%。结论:所建高效液相色谱方法可对复方丹参片中6种活性成分进行含量测定。

Objective： To establish HPLC methods for content determination of six kinds of active ingredients in compound DanShen tablets simultaneously. Method： Separated with VP-ODS C18 column （4.6 mm×150mm, 5μm, Shimadzu, Japan）; the mobile phase was 1% acetic acid （A）-accetonitrile （B） with gradient elution; detection wavelength： 263nm; column temperature： 40℃ ; flow rate： 1.0 mL/min. Result： Concentrations of six kinds of active ingredients indicated good linear relationships, r2 〉0.999; RSD 〈4.12%; the average recoveries were all higher than 98%. Conclusion： HPLC methods can be used to determine contents of six kinds of active ingredients in compound DanShen tablets.