中国板栗EST-SSR分子标记在栲树中的通用性分析

Transferabilityanalysis of EST-SSR markers of Castanea mollissimato Castanopsis fargesii

简单重复序列也称为微卫星分子标记,不仅在同属近缘种间具有良好的通用性,甚至在近缘属间也具有一定的通用性。本研究利用壳斗科基因组信息数据库中公布的中国板栗124对多态的EST-SSR引物在栲树中进行跨属(栗属到栲属)通用性研究,结果显示中国板栗EST-SSR引物在栲树中通用性和多态性分别为42.7%和56.6%;使用19对多态的EST-SSR引物对4个栲树自然居群的遗传多样性进行初步分析,结果显示栲树自然居群具有较高的遗传多样性(Na=6.105,Ho=0.563,He=0.621)。这些引物为栲树群体遗传学的深入研究提供了有力工具。

Simple sequence repeats（SSR）, also known as microsatellite molecular markers, have been cross-amplified successfully in closely related species of the same genus and even across genera within the same family. In the present study, 124 primer pairs of polymorphic EST-SSR originally developed from Castanea mollissima were cross-amplified in Castanopsis fargesii. Results indicated that 42. 7% of Castanea mollssima EST-SSR primers were successfully cross-amplified in Castanopsis fargesii and 56.6% were polymorphic. The genetic diversity of 4 populations of C. fargesii were investigated with polymorphic EST-SSRs, preliminary results showed that C. fargesii possessed high levels of genetic diversity（Na=6. 105,Ho=0. 563,He=0. 621）. These polymorphic EST-SSR primers would provide a powerful tool for further investigation on population genetics of C. fargesii.