桑树肌动蛋白actin基因全长序列的克隆与分析

Cloningand sequence analysis of full length of mulberry(Morus alba) actin gene

肌动蛋白在植物的各种生理活动中起着重要作用,是研究基因表达与调控模式的内标参考。通过染色体步移方法获得了桑树肌动蛋白actin基因1612bp的序列,该基因CDS长1312bp(GenBank登录号:HM623866),编码377个氨基酸残基,与水稻、葡萄等的同源基因的序列一致性在90%以上。基因内含子的数目及其在基因组上的位置也与水稻、葡萄等的相似。对来自不同物种的24个肌动蛋白基因进行聚类分析的结果显示,基因被分为ClassⅠ和ClassⅡ两个明显的亚群。

As a standard reference model of gene expression and regulation, actins play important roles in plants. In this study,the full length 1 612 bp of actin gene from mulberry was cloned by means of chromosome walking. Its CDS was 1 312 bp in length, encoding 377 amino acid residues. Homology analysis of the deduced amino acids showed above 90% identity with the actins from rice and grape,et al. The number and site of introns was conservative with rice and grape,too. Cluster analysis of actins from 24 species indicated that these genes were divided into two subgroups of Class I and Class II.