秋水仙素对蚕豆胚根生长的影响及多倍体诱导效应分析

Influence of colchicine on radicle growth of Vicia fabaand the polyploid inductive effect

筛选秋水仙素诱导蚕豆胚根多倍体的最适诱导处理组合并分析其诱导效应。以秋水仙素5个浓度(0.025%、0.050%、0.100%、0.150%、0.200%)和4个诱导时间(12、24、48、60h)正交组合(蒸馏水处理为对照)分别处理蚕豆胚根。以根尖诱导率和胚根膨大率的显著性差异确定蚕豆胚根多倍体的最适诱导处理组合,并以胚根细胞染色体数目和幼叶气孔数目的变化对诱导效果进行鉴定,分析其幼苗期的胚根数目和幼苗长度。结果表明:秋水仙素最适诱导时间为48h和浓度为0.100%;气孔数量在诱导时间和诱导浓度之间均有显著差异,但幼苗期的胚根数量和幼苗长度在不同的诱导时间之间无显著差异,诱导浓度之间差异显著。

In order to test the optimum induction combination （time and concentration）, and analyse the inductive effects of colchicine induced the radicle polyploid in Vicia faba. The radicle cells were treated with the colchicine of five concentrations （0. 025% ,0. 050% ,0. 100% ,0. 150% ,0. 200% ,respectively） combining various culture time （12,24, 48,60 h,respectively）. And the radicle cell were treated with distilled water simultaneously, which were taken as the control. The optimum induction combination induced by colchicine was confirmed by significant difference of the root tip inductivity and the radicle expanding ratio. And the chromosome number of radicle cells and stoma number of seedling were detected to identify the induction effect. The radicle amount and seeding height were analized subsequently. The optimum inductive condition was 48 h combining colchicine concentration＇of 0. 100%. There were significant differences in stoma number between inducted time and inducted concentration, however, the radicle number and height of V. faba seedling showed significant difference in inductive concentration but no significant difference in culture time.