黄瓜灰霉病拮抗菌的筛选鉴定及其抑菌特性研究

Identification and characterization of antagonistie strain biocontrol of cucumber Botrytis cinere and inhbition properties

从石家庄地区多个蔬菜大棚的根际土壤分离筛选到一株高效生防菌F1-2,对黄瓜灰霉病菌的抑菌圈直径高达33mm。根据菌株F1-2的形态特征、生理生化特性以及16SrDNA序列对其进行鉴定,初步确定为枯草芽孢杆菌(Bacillus subtilis)。采用生长速率法对拮抗菌的抑菌谱进行测定,其对所选的16种病原菌均有很好的抑制作用。实验还对拮抗菌F1-2的抑菌特性进行了研究,菌株连续传代至10代抑菌性能保持不变,菌株的发酵液对热和酸碱都比较稳定。该菌株的代谢产物具有广谱抑菌活性和较强的稳定性,具有开发成为灰霉病生防制剂的潜力。

An effective fungal strain F1--2 was isolated from rhizosphere soil of several greenhouses in Shiji- azhuang. The strain suppressed the growth of botrytis cinerea which was isolated from cucumber with the inhibition zone diameter of 33mm. The physiological and biochemical characteristics of the strain F1--2 and the identity of the 16SrDNA point out that this organism is Bacillus subtilis. Antimicrobial spectrum of the strain ,which could restrain the growth of 16 pathogens,was determined by growth rate method. The inhi- bition properties of strain F1--2 was researched in the experiment. After 10 generations,the antagonistic activity of the strain kept stable. Fermentation liquid of the strains have stability to heat, acid and base Metabolite of the strains was able to inhibit many kinds of pathogens and have high stability,which laid the foundation of development as botrytis cinerea suppressing Bio--control preparations.