川芎嗪对辐射所致小鼠氧化应激损伤的保护作用

Protective effects of ligustrazine against oxidative stress induced by irradiation in mice

目的:研究川芎嗪对辐射所致小鼠氧化损伤的预防和治疗作用。方法:采用^(60)Co-γ射线5 Gy全身单次照射小鼠造模,分别于照射前(预防)和照射后(治疗)每天腹腔注射川芎嗪31.2 mg/kg,连续给药10 d,并设生理盐水阴性对照组,检测各组小鼠肝组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GPx)及总抗氧化力(T-AOC)的变化。结果:与阴性对照组比较,^(60)Co-γ射线照射可显著增加小鼠肝组织中MDA的含量(P<0.05),降低SOD、GSH、GPx的活性(P<0.05),使肝组织T-AOC下降(P<0.05),但对CAT的活性无显著影响(P>0.05)。与照射组比较,照射前和照射后给予川芎嗪,均可降低小鼠肝组织MDA含量(P<0.05),使SOD、GSH、GPx的活性升高(P<0.05)、肝组织T-AOC升高(P<0.05),对CAT的活性仍无显著影响(P>0.05)。结论:川芎嗪具有较好的抗氧化作用,预防和治疗性用药均可降低辐射所致小鼠的氧化应激损伤。

OBJECTIVE：To study the preventive and therapeutic effect of ligustrazine against irradiation-induced oxidative stress.METHODS：Mice were exposed to 5 Gy single Co- 7 irradiation to induce oxidative stress,the concentration of ligustrazine for daily intraperitoneal injection was 31.2 mg/kg to prevent and treat liver injury before and after irradiation.The injection lasted 10 days.We measured the parameters of malondialdehyde（MDA）,superoxide dismutase enzyme（SOD）,catalase（CAT）,reduced glutathione（GSH）,glutathione peroxidase（GPx）,total antioxidation capability（T- AOC） activity in liver.RESULTS：Compared with negative control group,MDA was increased（P〈0.05）,the activities of SOD,GSH,GPx and T-AOC were decreased in the liver（P〈0.05） after irradiation,while the activity of CAT was not changed significandy（P〉0.05）.By using ligustrazine,compared with irradiation group,MDA was decreased（P〈0.05） and the activities of GSH,SOD,GPx,and T-AOC were increased significantly（P〈0.05）,whereas the activity of CAT was not changed significandy（P〉0.05）.CONCLUSION：Ligustrazine could reduce the oxidative injury induced by irradiation, either as prevention or treatment.