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Transwell技术模拟人类免疫缺陷病毒感染黏膜及其他屏障系统的应用

Modeling human immunodeficiency virus infection through mucosa and other barrier systems with Transwell
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摘要 近年来,由于Transwell小室能在体外模拟机体许多黏膜及生物屏障系统,如人生殖道黏膜、直肠组织黏膜以及血-脑屏障、血-视网膜屏障等,重现人类免疫缺陷病毒(HIV)感染机体的过程,对HIV感染机体的机制研究极为重要,因而被广泛应用。目前,尚无有效抗HIV疫苗问世,因此开发能阻断HIV性传播的药物是有效途径之一。应用Transwell技术深入研究HIV穿透黏膜及屏障系统感染机体的机制,也能为HIV防治药物的研发寻找新的作用靶点。本文就近年来Transwell技术在HIV感染黏膜及其他屏障系统中的应用作一综述。 Transwell is a device widely used for simulating mucosal and physiologic barrier systems, such as human reproductive tract mucosa, rectal mucosa, blood-brain barrier and blood-retina barrier in vitro. This device has been used to study the process of human immunodeficiency virus （HIV） entering into the body and to help us to illustrate the mechanism of HIV infection. The system is also being used to facilitate the discovery of new vaccines and drugs for HIV. This review covers the current progresses of Transwell applications in HIV-related studies.
作者 林光正 裘佳寅 刘叔文 李琳
机构地区 南方医科大学第一临床医学院 南方医科大学药学院
出处 《微生物与感染》 2012年第2期105-110,共6页 Journal of Microbes and Infections
基金 国家自然科学基金(30801412)
关键词 Transwell技术 人类免疫缺陷病毒 性传播 黏膜感染 滤泡树突细胞 屏障系统 Transwell Human immunodeficiency virus Sexual transmission Mucosal infection Folliculardendritic cell Barrier system
分类号 R512.91 [医药卫生—内科学]
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参考文献38

  • 1UNAIDS. 2010 Report on the global AIDS epidemic [R]. http://www, unaids, org/globalreport/Global report, htm.
  • 2Miller CJ, Li Q, Abel K, Kim EY, Ma ZM, Wietgrefe S, La Franco-Scheuch L, Compton L, Duan L, Shore MD, Zupancic M, Busch M, Carlis J, Wolinsky S, Haase AT. Propagation and dissemination of infection after vaginal transmission of simian immunodeficiency virus[J]. J Virol, 2005, 79(14): 9217-9227.
  • 3杨瑜,张晓燕.人体黏膜活组织模型在HIV-1性传播途径感染中的应用[J].生物工程学报,2011,27(2):172-179. 被引量：1
  • 4Shattock ILl, Griffin GE, Gorodeski GI. In vitro models of mucosal HIV transmission [J].Nat Med, 2000, 6(6): 607-608.
  • 5Gupta P, Collins K, Patterson B, Naus G, Landers D. Reply to ' In vitro models of mucosal HIV transmission' [J]. Nat Med, 2000, 6(6): 607-608.
  • 6Collins KB, Patterson BK, Naus GJ, Landers DV, Gupta P. Development of an in vitro organ culture model to study transmission of HIV-1 in the female genital tract[J]. Nat Med, 2000, 6(4):475-479.
  • 7Shen R, Drelichman ER, Bimczok D, Ochsenbauer C, Kappes JC, Cannon JA, Tudor D, Bomsel M, Smythies LE, Smith PD. GP41-specific antibody blocks cell-free HIV type 1 transcytosis through human rectal mucosa and model colonic epithelium [J]. J Immunol, 2010, 184 (7): 3648-3655.
  • 8Nazli A, Chan O, Dobson-Belaire WN, Ouellet M, Tremblay MJ, Gray-Owen SD, Arsenault AL, Kaushic C. Exposure to HIV-1 directly impairs mucosal epithelial barrier integrity allowing microbial translocation [J]. PLoS Pathog, 2010, 6(4): e1000852.
  • 9Bai L, Zhang Z, Zhang H, Li X, Yu Q, Lin H, Yang W. HIV-1 Tat protein alter the tight junction integrity and function of retinal pigment epithelium., an in vitro study [J]. BMC Infect Dis, 2008, 8: 77.
  • 10Sivard P, Berlier W, Picard B, Sabido O, Genin C, Misery L. HIV-1 infection of Langerhans cells in a reconstructed vaginal mucosa[J].J Infect Dis, 2004, 190(2) : 227-235.

二级参考文献49

  • 1姜拥军 康辉 等.体外转录HIV－1 RNA模板及在HIV－1病毒检测中的应用[J].中国医科大学学报,2000,29:130-130.
  • 2Beyrer C. Global prevention of HIV infection for neglected populations: men who have sex with men. Clin Infect Dis, 2010, 50(Suppl 3): S108-113.
  • 3Ministry of Health of the People's Republic of China, Update on the HIV/AIDS Epidemic in China. ~-12-02. http://www.moh.gov.cn.
  • 4Miller C J, Li Q, Abel K, et al. Propagation and dissemination of infection after vaginal transmission of simian immunodeficiency virus. J Virol, 2005, 79(14): 9217-9227.
  • 5Harrison RG, Greenman MJ, Mall FP, et al. Observation on the living developing nerve fiber. Anat Rec, 1907, 1(5): 116-128.
  • 6Wallace GS, Cheng-Mayer C, Schito ML, et al. Human immunodeficiency virus type 1 nucleocapsid inhibitors impede transinfection in cellular and explant models and protect nonhuman primates from infection. J Virol, 2009, 83(18): 9175-9182.
  • 7Fischetti L, Barry SM, Hope TJ, et al. HIV-1 infection of human penile explant tissue and protection by candidate microbicides. Aids, 2009, 23(3): 319-328.
  • 8Herrera C, Cranage M, transcriptase inhibitors McGowan I, et al. Reverse as potential colorectal microbicides. Antimicrob Agents Chemother, 2009, 53(5) 1797-1807.
  • 9Hladik F, Sakchalathorn P, Ballweber L, et al. Initial events in establishing vaginal entry and infection by human immunodeficiency virus type-1. Immunity, 2007, 26(2): 257-270.
  • 10Fletcher PS, Elliott J, Grivel JC, et al. Ex vivo culture of human colorectal tissue for the evaluation of candidate microbicides. AIDS, 2006, 20(9): 1237-1245.

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