广州管圆线虫感染小鼠脾脏T淋巴细胞亚群和细胞因子的变化

Alteration of T-cell subtype and cytokine in spleen of mice infected with Angiostrongylus cantonensis

目的观察小鼠感染广州管圆线虫后脾脏T淋巴细胞亚群及其细胞因子分泌水平的变化。方法用从感染褐云玛瑙螺分离的广州管圆线虫第3期幼虫感染BALB/c小鼠,感染后第19d及第25d分批处死小鼠,分离脾细胞,使用细胞内细胞因子荧光染色方法,用流式细胞仪检测脾脏淋巴细胞中的T细胞亚群的含量,用双抗夹心ELISA法检测脾细胞培养上清细胞因子的浓度。结果与对照组比较,感染小鼠脾脏CD4+、CD4+IL-4+T淋巴细胞比例显著上升,而CD4+IL-17+、CD4+IFN-γ+T淋巴细胞比例显著下降;脾细胞培养上清细胞因子IL-4水平明显升高,而IL-17水平明显下降。同时,感染程度和感染时间等因素也可造成脾脏T淋巴细胞亚群及其细胞因子分泌水平的变化。结论小鼠感染广州管圆线虫后,表现以脾脏CD4+T淋巴细胞大量增殖和Th2型淋巴细胞极化为特点。

To investigate the changes of T-cell subtypes and cytokine level in spleen of mice infected with Angiostrongylus cantonensis,BALB/c mice were infected with third-stage larva of Angiostrongylus cantonensis from Achatina fulica.The splenocytes of mice were collected and cultured on the 19th and 25th day post-infection.The subtypes of T-cells were detected by Flow Cytometry with fluorescence staining and the cytokines in cultured supernatants of splenocytes were assayed by the method of ELISA.Results showed that the percentages of CD4＋ and CD4＋IL-4＋T cells in splenocytes of infected mice were much higher than those in normal control mice,however,the percentages of CD4＋ IL-17 ＋ and CD4＋IFN-γ＋T cell were much lower.In comparison with normal control group,the concentration of IL-4 in cultured supernatants of splenocytes in infected groups increased significantly,while that of IL-17 decreased significantly.In addition,the factors,such as the number of larva infected and days after infection,could influence the T-cell subtypes and their cytokine levels in spleen.It is evident that the CD4＋ T lymphocytes of spleen in the mice infected with Angiostrongylus cantonensis proliferate markedly and polarize to Th2 phenotypes.