牙龈卟啉单胞菌侵入对人血管内皮细胞分泌MCP-1影响的研究

Expression of MCP-1 in vascular endothelial cells invaded by Porphyromonas gingivalis

目的通过研究牙龈卟啉单胞菌(porphyrmonas gingivalis,P.g)侵入对人脐静脉内皮细胞(human umbilical vein endo-thelial cell,HUVEC)分泌单核细胞趋化蛋白(MCP-1)的影响,了解P.g对其趋化功能的影响。方法建立P.g侵入HUVEC的体外模型,采用酶联免疫吸附法(ELISA)研究P.g381和P.g33277菌株侵入HUVEC 6、24 h后的培养上清液中MCP-1浓度。结果 ELISA结果显示当P.g381侵入HUVEC 6、24 h和P.g33277侵入HUVEC 6 h时,HUVEC分泌的MCP-1水平升高(P<0.01);P.g33277侵入HUVEC 24 h时,HUVEC分泌的MCP-1水平恢复最初水平(P=0.46);P.g381诱导HUVEC表达MCP-1的水平高于P.g33277(P<0.01)。结论 P.g侵入HUVEC后可促进其表达MCP-1,从而上调其趋化功能,在牙周炎与心血管疾病的相关性中可能发挥作用。

Objective To investigate the expression of monocyte chemoattractant protein-1（MCP-1） in human umbilical vein endothelial cells（HUVEC）invaded by porphyrmonas gingivalis（P.g）.Methods The models of invasion of P.g into HUVEC were established in vitro.HUVEC were infected with either P.g381 or P.g33277,then the expression of MCP-1 in supernatant was tested by ELISA.Results The expression of MCP-1 was signicantly upregulated in P.g381-infected HUVEC at 6h and 24h and in P.g33277-infected HUVEC at 6h（P〈0.01）.The upregulation ability of P.g381 was significantly greater than P.g33277（P〈0.01）.Conclusions These data demonstrate that the infection of P.g can increase the expression of MCP-1 and chemotactic function of HUVEC,which may play a role in the relationship between periodontitis and cardiovascular diseases.