非小细胞肺癌组织中E1AF、MMP-7、uPA的表达及其临床意义

Expressions and clinical significances of E1AF,MMP-7 and uPA in non-small cell lung cancer

目的检测非小细胞肺癌(NSCLC)组织中E1A因子(E1AF)、基质溶解素(MMP-7)、尿激酶纤溶酶原激活物(uPA)的表达及其与临床病理特征之间的关系。方法采用免疫组织化学技术检测56例NSCLC组织及其癌旁配对正常肺组织中MMP-7、uPA蛋白的表达,采用RT-PCR方法检测E1AFmRNA的表达。结果与癌旁配对正常肺组织相比,NSCLC中E1AFmRNA、MMP-7和uPA表达明显升高(P<0.01),E1AF表达与MMP-7及uPA的表达分别呈正相关(P<0.05);MMP-7与uPA表达无显著相关性(P>0.05);E1AF、MMP-7、uPA表达均与组织类型无关,E1AF、MMP-7与分化程度有关,uPA与肿瘤大小有关,三者均与TNM合并分期和淋巴结转移密切相关(P<0.05)。结论 E1AF、MMP-7和uPA在NSCLC组织中的表达与肿瘤的浸润、转移有关,E1AF可能在转录水平上调控MMP-7和uPA的表达,E1AF基因可作为肺癌基因诊断和治疗的新靶点,联合检测有助于判断患者的预后。

Objective To detect the expressions of early region 1A factor （EIAF）, matrilysin （MMP-7） and uroki- nase-type plasminogen activator （uPA） in non-small cell lung cancer tissues （NSCLC）, and to study their relationships with the clinical and pathological features. Methods The expressions of MMP-7 and uPA in 56 cases of NSCLC tis- sues and paired normal lung tissues were detected by SP immunohistochemical staining and examination on the expres- sion of E1AFmRNA by RT-PCR assay. Results The expressions of E1AFmRNA, MMP-7 and uPA in the tissues of NSCLC were significantly higher than those in the paired normal lung tissues （P 〈 0.01 ）. There were also positive cor- relations between the expressions of E1AF and MMP-7, uPA in NSCLC（P 〈0.05）. The expressions of MMP-7 and uPA had no significant correlation（ P 〉 0.05 ）. The expression levels of E1AF, MMP-7 and uPA were closely related with clinical merger stages and lymph node metastasis, but had no relationship with histological classification. E1AF and MMP-7 were closely related with differentiations, and uPA was closely related with tumor size （ P 〈 0. 05 ）. Conclusion Abnormal expressions of EIAF, MMP-7 and uPA may play important roles in the infiltration and metasta- sis in NSCLC; E1AF may regulate the expressions of MMP-7 and uPA at transcription level; E1AF gene can be used as a new target gene for diagnosis and treatment of lung cancer; the united detection of E1AF, MMP-7 and uPA expres- sions mav be heloful to evaluate the nrognosis of NSCLC.