葛根素预防雌激素缺乏性骨质疏松的机制探讨

Effect of Puerarin on the Osteoporosis Resulted from Ovariectomy in Rats

目的观察葛根素对去卵巢大鼠股骨骨密度、颌骨骨密度和血清中肿瘤坏死因子(TNF-α)、C反应蛋白(CRP)及雌二醇(E2)水平的影响。方法 3月龄雌性SD大鼠随机分为假手术组(sham group)、单纯去势组(OVX group)、去势+雌二醇治疗组(OVX-estrogen group)和去势+葛根素治疗组(OVX-puerarin group)。给药3个月后测量各组大鼠股骨和颌骨骨密度,血清中TNF-α和CRP及雌二醇(E2)水平。结果 OVX-puerarin组较sham组体重明显增加(P<0.01);较OVX-estrogen组的TNF-α(P<0.01)、CRP(P<0.05)及E2(P<0.01)水平显著下降;其下颌骨和股骨远端骨密度与OVX组、sham组之间无显著性差异,但其股骨近端骨密度较OVX-estrogen组明显增加(P<0.05)。结论葛根素作为抗炎因子,能够显著降低TNF-α和CRP的水平,但并不会提高雌激素的水平;葛根素对卵巢切除所致的骨质疏松症有一定的治疗作用,但会明显提高机体体重。

Objective To comparatively evaluate the therapeutic effect of puerarin on bone mineral density of the mandible and femur in ovariectomized rats,and to investigate the association between TNF-α,CRP and E2.Methods 40 female 3-month old SD rats were divided into 4 groups randomly： sham operation group（sham）,ovariectomized（OVX） group,ovariectomized group with injection of estrogen（OVX-estrogen） and ovariectomized group with injection of puerarin.After 3 months,TNF-α,CRP and E2 in the serum were tested.Meanwhile,the bone mineral density of the mandible and femur was measured.Results The body weight of OVX-puerarin group was signficantly higher than that of sham group（P 0.01）.Compared with OVX-estrogen group,the TNF-α,CRP and E2 serum level of OVX-puerarin group was significantly lower（respectively P 0.01,P 0.05,P 0.01）.As to mandible and distal femur bone mineral density,there were no significant difference among OVX-puerarin group,sham group and OVX group,but the proximal femur bone mineral density of OVX-puerarin group was significantly higher than that of OVX-estrogen group（P 0.05）.Conclusion As an anti-inflammatory factor,puerarin can significantly reduce the level of TNF-α and CRP in the serum,but can＇t promote the level of E2 in the serum in ovariectomized rats.Puerarin has some therapeutic effect on the ovariectomy-induced osteoporosis,but can significantly increase the body weight of ovariectomized rats.