SIVmac239感染恒河猴急性期回肠派氏淋巴结中淋巴细胞亚群的变化

The Changes of Lymphocytes from Ileum Peyers Pacth from Rhesus Monkeys at the Earliest Period with SIVmac239 Infection

目的分析SIVmac239感染早期中国恒河猴回肠派氏淋巴结淋巴细胞数量及亚群的变化,探讨这些变化与疾病进展的可能关系。方法以静脉注射SIVmac239制备恒河猴AIDS模型,对回肠派氏淋巴结进行CD4和CD8免疫组化标记,分离Peyer’s集合淋巴结淋巴细胞,分别标记CD3、CD4、CD8、CD28、CD95单克隆抗体,以流式细胞仪检测T细胞及其亚群的表达情况。结果 SIVmac239感染急性期中国恒河猴Peyer淋巴结中CD4+/CD8+比值持续下降,记忆性细胞比例升高,但Peyer淋巴结形态及CD4+T细胞数量未见明显变化,CD8+T细胞从第5天开始持续升高。结论 SIVmac239感染急性期,中国恒河猴回肠派氏淋巴结形态及CD4+T细胞数量基本维持,向记忆性细胞的转化增加,但是CD4+/CD8+比值下降。

Objective To analyze the changes of lymphocytes from ileum Peyers pacth from Chinese rhesus monkeys at acute period with SIVmac239 infection.Methods 9 rhesus monkeys were used and 5 were infected with SIVmac239 via intravenous injection.The tissues or lymphocytes from ileum Peyers pacth lymph nodes from rhesus monkeys at acute period were collected and analyszed with CD4＋,CD8 immunohistochemistry and flow cytometry T-cell subsets labeling with CD3,CD4,CD8,CD28,CD95 monoclonal antibodies respectively.Results Obviously declined CD4＋/CD8＋ ratio and increased memory cells（CD4 ＋ CD8 ＋） ratio and CD8＋ T cells were detected in Peyers lymph nodes,but CD4 counts had no significant change in Peyers lymph nodes.Conclusion Declined CD4＋/CD8＋ ratio and increased memory cells（CD4 ＋ CD8 ＋） ratio were found in Peyers lymph nodes from rhesus macaques at acute period with SIVmac239 infection,however,the meaning and possible affection to disease need further study.