高效液相色谱法测定化感小麦根系分泌的酚酸类物质

Determination of 10 Phenolic Acids in Root Exudates of Wheat by High Performance Liquid Chromatography

【目的】建立高效液相色谱(HPLC)测定小麦根系分泌物中的10种酚酸类化合物的方法。【方法】选用Ex-tended-18 C色谱柱(250mm×4.6mm,5μm),以甲醇与0.1%磷酸混合液作为流动相进行梯度洗脱,25min内各种酚酸实现有效分离。【结果】建立的方法成功运用于小麦根系分泌物酚酸类化合物的检测,回收率为88.14%～102.43%。【结论】该方法简便、快速、灵敏,准确,适用于沙土培养的小麦中各种酚酸类物质含量的同时测定。

[Objective] the objective was developed a method for measuring 10 phenolic acids by RP-HPLC in root exudates of wheat. [Method] Chromatographic separation was performed with Extended-^18C column （250 mm× 4.6 mm, 5 μm） by using the mobile phase gradient elution of methanol --0.1% phosphoric acid mixture, under the optimum conditions, 10 compounds were successfully separated within 25 min. [Results] The developed method was used to measure and analyze the 10 phenolic acids in root exudates of wheat. The average recoveries were 88. 14% 102.43%. [Conclusion] This method is simple, rapid, sensitive, and accurate, and it is appropri- ate for determining the phenolic acids in root exudates of wheat simultaneously.