孔源性视网膜脱离视网膜下积液中神经元特异性烯醇酶和S100B浓度的研究

Study of NSE and S100B concentration in subretinal nuid of rhegmatogenous retinal detachment

目的通过对视网膜下液(SRF)中神经元特异性烯醇酶(NSE)和S100B蛋白浓度的测定,评估孔源性视网膜脱离(RRD)时视网膜的神经损伤程度。方法 92份视网膜下液的样本取自孔源性视网膜脱离患者的外路手术中,同时抽取患者血清样本作为实验组,另外抽取19份角膜捐献病人的玻璃体和20份正常人的血清样本作为对照组,采用双夹心酶联免疫吸附法(ELISA)测定实验组和对照组中NSE和S100B的浓度。结果本实验视网膜下液中的NSE和S100B蛋白浓度与这些患者及正常人血清NSE和S100B蛋白浓度的差异有统计学意义(P<0.05);在病程和网脱范围间的差异均有统计学意义(P<0.05)。结论在RRD的发生发展过程中存在神经元和神经胶质细胞的损伤,这种损伤随网脱范围的增大而增大,随病程延长而增大,且在2周内增长最为迅速。

Objective To determine the concentration of Neuron-Specific Enolase （NSE）and S100B in subretinal fluid（SRF）and to assess the neural injuries in rhegmatogenous retinal detachment （ RRD }. Methods SRF specimen and blood serum were collected during the surgery in ninety-two cases with rhegrnatogenous retinal detachment served as experimental group. Nineteen vitreous collected during cornea procurement from deceased patients and twenty normal serum served as control group. These specimens were detected using enzyme-linked immunosorbent assy { ELISA }. Results NSE and SIOOB centration＇s difference of these patients who have rhegmatogenous retinal detachment, between in the subretinal fluid and the serum, was statistically significant （ P 〈 0. 05 ）. The difference of NSE and S100B concentration in the subretinal fluid between retinal detachment range and duration was statistically significant （ P 〈 0. 05 ）. Conclusion Neuron and gliacyte injuries exist in the subretinal fluid of rhegmatogenous retinal detachmento These injuries enhance with retinal detachment range and duration , especially within two weeks.