菜园根际土壤解磷真菌的分离筛选及初步鉴定

ISOLATION AND SCREENING OF PHOSPHATE-SOLUBILIZING FUNGI FROM THE RHIZOSPHERIC SOIL AND ITS PRILIMINARY IDENTIFICATION

为提高土壤磷利用率及微生物修复污染环境提供参考依据,本研究采用平板涂布法,从不同蔬菜根际土壤中分离得到11株降解有机磷和无机磷的解磷真菌。根据透明圈直径和菌落直径比值的大小,从中筛选到3株分解无机磷较强的菌株WP-2、WP-5、WP-7;并进一步通过液体摇瓶培养复筛,确定其中的WP-5菌株分解无机磷能力最强,其解磷能力达66.88mg/L。经初步鉴定,WP-2菌株属于木霉属(Trichoderma sp)、WP-5菌株属于青霉属(Penicillium sp)、WP-7菌株属于小克银汉霉属(Cunninghamella sp)。

In order to improve the utilization ratio of soil phosphorus and provide the reference for microbial remediation pollution environment, eleven strains of organic and inorganic phosphate-solubilizing fungi were isolated from different vegetables rhizospheric soil by spread plate method. Three fungi strains WP-2, WP-5 and WP-7 with higher inorganic phosphate-solubilizing ability were obtained according the ratio of hyaline circle diameter and colony diameter, and then the strain WP-5 with the highest inorganic phosphate-solubilizing ability was screened out by shake culture experiment, its ophosphate-degradation ability reached to 66.88mg/L. Three strains were selected and identified as Trichoderma sp., Penicillium sp. and Cunninghamella sp.