SD大鼠脊髓损伤前后外周血T淋巴细胞亚群的变化

The change of T lymphocyte subsets in peripheral blood of SD rats with acute spinal cord injury

目的:探讨SD大鼠脊髓损伤(spinal cord injury,SCI)前后外周血T淋巴细胞亚群的动态变化。方法:取22只健康SD大鼠(雌雄各半),采用NYU脊髓损伤仪制作重物坠落SCI模型。于SCI前、SCI后1、3、5、7和14 d采外周血,用流式细胞术检测外周血淋巴细胞中CD3+T、CD3+CD4+T和CD3+CD8+T细胞比例的变化。结果:SCI前与SCI后1、7和14 d CD3+CD8+T细胞比例均无明显变化(P>0.05),而SCI后第3、5 d均高于SCI前14 d(P<0.01)。SCI后1、3、5 d及7 d的CD3+T细胞、CD3+CD4+T细胞比例,以及CD3+CD4+/CD3+CD8+比值均明显低于SCI前(P<0.01)。在SCI后14 d,上述各T细胞亚群的比例均恢复到术前水平,两时间点之间比较差异均无统计学意义(P>0.05)。结论:SCI后,SD大鼠总T细胞和CD4+T细胞明显降低,CD3+CD4+/CD3+CD8+下降。提示SCI后的早期阶段,可能存在T细胞功能的异常。

Objective ： To investigate the change of T lymphocyte subsets in peripheral blood of SD rats with acute spinal cord injury （SCI）. Methods： Twenty two SD rats （ 11 each male and female） were used to establish SCI model using NYU Spinal Cord Contusion Impactor System. The CD3 ＋ , CD3 ＋ CD4 ＋ and CD3 ＋ , CD8 ＋ T cells, and the proportion of CD3 ＋ CD4 ＋ to CD3 ＋ CD8 ＋ T cells of peripheral blood of all rats were evaluated using flow eytometry at the time points of preinjury and days 1,3,5,7and 14 postinjury. Results ：The percentages of CD3 ＋ CD8 ＋ cells had no significant difference among before SCI, the l st,7th and 14th day after SCI, but the percentage of CD3 ＋ ,CD8 ＋ T ceils at the 3rd,Sth day was higher than at the 14th day after SCT and before SCI. The percentages of CD3 ＋ and CD3 ＋ CD4 ＋ T cells,and ratio of CD3 ＋ CD4 ＋ to CD3 ＋ CD8 ＋ T cells at days 1,3,5 and 7 post-injury were significantly lower than those of at preinjury （P 〈 0.01 ）. After 14 days postinjnry, all T lymphocyte subsets were restored to preoperative level, which were no differences with preinjury（ P 〉 0.05 ）. Conclusions ： After acute SCI, the total of T cells and CD4 ＋ T cells and the ratio of CD3 ＋ CD4＋ to CD3 ＋ CD8 ＋ T cells decrease, which suggest there may be T cell function abnormalities in the early stage of SCI in rat model.