猪附红细胞体病BALB/c小鼠模型的建立

Establishment of BALB/c mice model of swine Eperythrozoonosis

为了解现阶段延边地区猪附红细胞体的致病性,以注射地塞米松和摘除脾脏的BALB/c小鼠为实验动物,以腹腔注射方式人工感染猪附红细胞体,通过临床症状观察、血液涂片姬姆萨染色镜检及PCR方法对BALB/c小鼠感染猪附红细胞体情况进行鉴定,并进行血液常规检测。结果表明,注射地塞米松组、摘除脾脏组和正常感染组BALB/c小鼠在感染后8～10d相继达到高峰,正常感染组BALB/c小鼠在感染后第24天猪附红细胞体消失,注射地塞米松组在感染后第32天消失,而摘除脾脏组在试验结束时仍具有较高的感染率。经血液常规检测,各组BALB/c小鼠在感染猪附红细胞体后,白细胞总数显著升高(P<0.01),淋巴细胞总数、红细胞总数、血红蛋白含量均显著下降(P<0.05),红细胞比容显著下降(P<0.01)。因此,猪附红细胞体对低免疫力的BALB/c小鼠具有一定感染性,并表现出典型临床症状。

In order to understand the pathogenic of Eperythrozoon suis in Yanbian area, BALB/c mice were divided into 3 groups： BALB/c mice in group 1 were injected with dexamethasone, BALB/c rnice＇s spleens in group 2 were removed and BALB/c mice in group 3 were normal. The BALB/c mice were artificially infected with E. suis by intraperitoneal injection. BALB / c mice infected with E. suis were identified by clinical observation of symptoms, blood smear, Giemsa staining and PCR method. And routine blood test was carried out. The results showed that the infection rates of the 3 groups reached their peaks in 8- 10 days after infection. E. suis was disappeared in group 3 in the 24 th day after infection. E. suis was disappeared in group 1 in the 32 nd day after infection. However, the infection rates of the group 2 was still high. Routine blood test showed that after infected with E. suis in each group, the total number of white blood cells was significantly incre＇ased （P〈0.01）, the total number of lymphocyte, red blood cells and the level of hemoglobin were significantly decreased （P〈0.05）. Red blood cells and the hematocrit was significantly decreased （P〈0.01）. This test confirmed that BALB/c mice could be infected by E. suis and the infected mice showed typical clinical symptoms.