超高效液相色谱-串联质谱检测动物源性食品中头孢喹肟残留

The Study of Cefquinome Residues in Food of Animal Origin by UPLC-MS/MS

建立了动物源性食品中头孢喹肟残留量检测的超高效液相色谱-串联质谱法。样品经乙腈水溶液提取后,用C18固相萃取柱净化、浓缩。头孢喹肟残留量在10～500μg/L浓度范围内线性关系良好,r=0.9996。方法检测限为1 ng/g,定量限为5 ng/g。猪肌肉在5～100 ng/g空白添加浓度范围内平均回收率为57.7%～69.4%,RSD为6.68%～13.41%,猪肝在5～100 ng/g空白添加浓度范围内平均回收率为50.6%～58.6%,RSD为5.13%～12.42%,牛奶在5～40 ng/g空白添加浓度范围内中平均回收率为59.2%～65.3%,RSD为6.12%～13.26%。

An ultra performance liquid chromatography - tandem mass spectrometry （UPLC - MS/MS） method was established for the determination of cefquinome residues in food of animal origin. Samples were extracted by water and acetonitrile and cleaned up on C18 solid - phase extraction cartridges. Calibration curve was linear between 10 and 500μg/L, with the correlation coefficient, r =0. 9996. The recovery in porcine muscle was 57.7 % -69.4 % and RSD was 6.68 % - 13.41% among 5 ng/g -100 ng/g ,The recovery in porcine liver was 50.6 % -58.6 % and RSD was 5.13 % -12.42 % among 5 ng/g- 100 ng/g,The recovery in bovine raw milk was 59.2 % -65.3 % and RSD was 6.12 % -13.26 % among 5 ng/g -40 ng/g.