清肝和胃汤对大鼠胃溃疡胃黏膜的保护作用及机制研究

Protective effect of Qingganhewei decoction on gastric mucosa in rat gastric ulcer and its mechanism

目的探讨清肝和胃汤对大鼠胃溃疡的胃黏膜保护作用及机制,为该药的临床应用提供理论依据。方法清洁级SD大鼠60只,雌雄各半,随机分成正常对照组,模型组,西咪替丁组,清肝和胃汤高剂量组,中剂量组,低剂量组,除正常组外,其余各组大鼠均用100%的冰醋酸黏膜下注射,造成大鼠胃溃疡模型。结果清肝和胃汤治疗组和西咪替丁组均能较模型组明显减少大鼠胃溃疡指数(P<0.01)。清肝和胃汤高剂量组与西咪替丁组比较差异有统计学意义(P<0.01),中、低剂量组与西咪替丁组比较差异无统计学意义(P>0.05)。结论清肝和胃汤对慢性胃溃疡大鼠模型溃疡灶肉芽组织及溃疡灶表面黏膜生长具有明显的促进作用,呈剂量依赖性关系。

Objective To study the protective effects of Qingganhewei decoction on gastric mucosa in rat gastric ulcer and its mechanism, and to provide the theoretical basis for clinical application. Methods Sixty SD rats, half male and half female, were randomly divided into normal control group, model group, cimetidine group, Qingganhewei decoction high dose group, the Qingganhewei decoction medium dose group, Qingganhewei decoction low dose group. Except for the normal group, and the rest of the rats were using 100% of glacial acetic acid injection of the mucous membrane, causing the rat gastric ulcer models. Results Compared to model group, Qingganhewei decoction treatment group and cimetidine group all can significantly reduce rat gastric ulcer index （P 〈0.01）. Between Qingganhewei decoction high dose group and cimetidine group, there was more significant differences （P〈0. 01）. Between medium,low dose group and cimetidine group there is no significant differences （P〉0.05）. Conclusion Qingganhewei decoction has an obvious improving role in granulomatous and ulcer surface mucosa growth in chronic peptic ulcer rats, which shows a dose-dependent effect.