结肠早癌自体荧光内镜诊断系统研究 Ⅰ.结肠组织显微自体荧光图像分析

A STUDY ON AUTOFLUORESCENCE ENDOSCOPIC DIAGNOSTIC SYSTEM FOR EARLY COLONIC CANCER Ⅰ. MICROSCOPIC AUTOFLUORESCENCE IMAGING OF COLONIC TISSUES

目的 :探讨结肠正常组织和腺癌组织的显微自体荧光图像差异与肠壁不同结构层次的荧光强度差异。方法 :采用共聚焦显微自体荧光图像系统采集与分析结肠正常和腺癌组织的自体荧光图像。结果 :结肠正常组织自体荧光图像能清楚显示肠壁的三层结构 ,自体荧光主要来自于细胞间质和粘膜下层 ,上皮细胞的荧光强度很弱 ;腺癌组织的肠壁结构发生明显改变 ,荧光强度主要来自于粘膜下层 ;腺癌组织的显微自体荧光强度显著低于正常组织 (P <0 .0 5 ) ,表明自体荧光分析法用于大肠癌的诊断是一个可行的方法。

To investigate the difference of microscopic autofluorescence imaging of normal and adenocarcinoma colonic tissue, and the autofluorescence intensity difference among the colonic tissue layers. The microscopic autofluorescence in normal colonic tissues could be divided clearly into mucosa layer, submucosa layer and muscular layer. The major fluorophores in normal colon were located in the muscularis mucosa and submucosa layers, the intensity of epithelial cells was very weak. The tissue composition of the adenocarcinoma changes and the major fluorophores were located in the submucosa layers. The autofluorescence intensity of adenocarcinoma colon also decreased significantly compared with normal mucosa( P< 0.05). The significant differences in histological structures and microscopic fluorescence between normal and adenocarcinoma colonic tissues suggest that autofluorescence technique is a powerful means for early diagnosis of diseased colonic tissues.