颈交感神经切断对臂丛神经节神经肽Y含量及前肢痉挛影响的实验研究

Experimental study about contribution of sympathetic nerve amputation to neuropeptide Y content in brachial plexus and spasmodic muscles in foreleg

目的探讨臂丛神经内神经肽Y含量与痉挛骨骼肌之间的关系。方法将16只右侧大脑皮层损伤模型Wis-tar大白鼠随机分成两组(各8只),实验组行颈上节交感神经切断术,对照组不予任何处理。于术后第20天对各动物相同切取相同部位左臂丛神经组织,用免疫组织化学方法检测其神经肽Y(NPY)百分率并做定量分析。同时观察左前肢术后神经症状和进行屈肘肌群痉挛评定。结果实验组大鼠左臂丛神经内NPY含量明显低于对照组,差异有统计学意义(P<0.05);大部分实验组动物患肢神经症状改善。结论交感神经切断术治疗大脑损伤性肌痉挛的机制可能与降低神经局部的NPY含量有关。

Objective To study the relationship between the neuropeptide Y content （NPY） in brachial plexus and spasmodic muscles. Methods 16 Wistar rat models that were injured on right cerebral cortex were diveded into two groups （8 rats in each group）： the experiment group were operated superior cervical sympathectomy, while the control group were not treated. The same left braehial plexus tissue of each rats were removed through sympathectomy on 20th day; then the NPY percentage was examined and quantitative analysis was made through immunohistochemical method. Meanwhile, the nerve symptom was observed and the spasm of flexion elbow muscles on left foreleg was assessed after sympathectomy. Results The NPY content of left braehial plexus was significantly lower in experiment group than control group （P 〈 0.05）. The nerve symptom of the affected limbs was improved in most of experiment group rats after sympathectomy. Conclusion The treatment mechanism of sympatheetomy for myospasm resulting from cerebrum injury may be related to the decrease of local content of NPY.