胃癌患者血清中miR-106a的表达及其临床意义

Detection of miR-106a in serum of patients with gastric cancer using a real-time quantitative RT-PCR platform and its clinical significance

目的探讨胃癌患者血清miR-106a表达及其与临床病理特征之间的关系。方法采用实时定量RT-PCR方法检测21例胃癌患者及10例健康人血清中miR-106a表达的水平。结果实时定量RT-PCR方法能够检测到血清样本中微量miRNA的表达。21例胃癌患者miR-106a表达水平的中位数为0.34(0.17～0.69),10例健康人为0.63(0.27～1.0),两者差异有统计学意义(P=0.002)。在21例胃癌患者中,血清miR-106a表达水平与肿瘤分化程度有关,在低分化胃癌中的表达水平明显高于中分化胃癌(P=0.01);血清miR-106a表达水平亦与血清CA724相关(P=0.03),而与分期、性别、年龄无关(P>0.05)。结论采用实时定量RT-PCR方法检测血清中miR-106a的表达敏感可靠。血清miR-106a作为新的生物学指标对于胃癌的诊断具有一定的价值。

Objective To investigate the expression level of miR-106a in serum of patients with gastric cancer and its clinical significance. Methods Real-time quantitative RT-PCR （qRT-PCR） was performed in serum of 21 gastric cancer patients and 10 normal controls. Results Small amount of miR-lO6a was detected in serum using a qRT-PCR platform. The median expression level of miR-106a was 0. 34（0. 17-0. 69）in 21 gastric cancer patients and 0. 63（0. 27-1.0）in 10 normal controls. Serum miR-106a expression level in 21 patients was significantly lower than that in normal controls （P = 0. 002）. Serum miR-106a expression was significantly associated with tumor differentiation（ P =0. 01 ） and serum CA724（ P = 0. 03 ）. No correlation was found between serum miR-106a expression level and tumor stage, sex or age （ P 〉 0.05 ）. Conclusion The qRT-PCR assay is reliable and sensitive for detecting the expression of miR-106a. Our data confirm a role for serum miR-106a as a novel cancer biomarker in gastric cancer.