病毒性肝炎患者肝内一氧化氮合酶活性检测及意义

Detection of Hepatic Nitric Oxide Synthetase in Patients with Viral Hepatitis and Significances

目的:探讨NOS在病毒性肝炎患者肝组织中的活性及意义。方法:用组织化学染色法检测20例病毒性肝炎患者肝穿组织内NOS,并检测积分光密度用于分析NOS活性。结果:NOS显色强度与肝组织病变相关,积分光密度均值慢性肝炎高于急性肝炎(P<0.05),重型肝炎高于慢性肝炎(P<0.05)。NOS阳性细胞主要分布于肝细胞及静脉内皮细胞。结论:NOS显色结果可能反映肝组织损伤程度。

Aim:To research the activity and significance of Nitric Oxide Synthetase (NOS) in the patients with viral hepatitis. Methods: The hepatic NOS in 20 patients were measured by histochemistry stain and the activity of NOS were analysis by integral luminous density. Results: The intensity of NOS stain was related to hepatic damages. The integral luminous density of chronic hepatitis was higher than acute hepatitis (P<0. 05), severe hepatitis was higher than chronic hepatitis (P<0. 05). The NOS positive cells most frequently apperenced in hepatocyte and vein endothelial cells. Conclusion: NOS stain may reflect the degree of hepatic damage.