丹酚酸A对成纤维细胞活力、增殖及胶原合成的影响

Effect of Salvianolic Acid-A on NIH/3T3 Fibroblast Viability, Proliferation and Collagen Synthesis

目的:探讨丹酚酸A对成纤维细胞增殖及胶原合成的影响。方法:常规培养NIH/3T3成纤维细胞,10^(-4)～10^(-7)mol/L丹酚酸A温育亚单层细胞22h。[~3H]-TdR掺入法测定细胞增殖,[~3H]-proline掺入法测定细胞活力,[~3H]-proline掺入、胶原酶消化法测定细胞内外胶原生成率。结果:10^(-4)～10^(-6)mol/L丹酚酸A抑制细胞增殖,除10^(-4)mol/L丹酚酸A组细胞[~3H]-proline掺入量下降,有一定细胞毒性外,其余各组均有促进细胞活力的趋势,以10^(-6)mol/L组最为明显。10^(-4)～10^(-7)mol/L丹酚酸A抑制细胞内胶原合成率,以10^(-6)mol/L作用稍强,但对细胞外胶原的分泌均无明显影响。结论:丹酚酸A能抑制成纤维细胞增殖及细胞内胶原合成,体外最佳作用浓度为10^(-6)mol/L。

Aim: To study effect of Salvianolic Acid-A (SA-A) on NIH/3T3 fibroblast functions such as viability, proliferation and collagen synthesis. Methods: NIH/3T3 fibroblasts were cultured with regular method, and incubated with 10-4-10-7mol/L SA-A for 22h. The cell viability was assayed by [3H]-proline incorporation, cell proliferation by [3H] -TdR incorporation, cell collagen synthesis rate was measured with [3H] -proline incorporation collagenase digestion. Results 10-4-10-6mol/L SA-A could inhibited fibroblast except 10-4 mol/1 SA-A which decreased cell viability, and 10-6mol/L had the best effect among three groups also 10-5-10-5mol/L SA-A inhibited intracellular collagen synthesis. 10-5mol/L showed a better effect. but both 10-5-10-6mol/L SA-A had no significant influence on extracelluar collagen secretion. Conclusion : SA-A could inhibited fibroblast proliferation and intracellular collagen synthesis, the best drug concentration perhaps was 10-6mol/L in vitro.