血管紧张素原M235T基因多态性与老年高血压及其合并糖尿病的相关性

Correlation of angiotensinogen gene polymorphism,elderly hypertension and elderly patients with hypertension and diabetes

目的探讨血管紧张素原(AGT)M235T基因多态性与老年高血压及其合并糖尿病的关系。方法采用聚合酶链反应(PCR)及限制性片段长度多态性分析法对60名健康老年对照组、105例老年高血压组、38例老年高血压合并糖尿病组进行M235T基因多态性检测。结果 MT、TT基因型和T等位基因频率在老年高血压组分别为0.371、0.457和0.643,老年高血压合并糖尿病组分别为0.289、0.632和0.776,均明显高于健康老年对照组的0.167、0.133和0.217(均P<0.05);老年高血压组与老年高血压合并糖尿病组患者的MT、TT基因型差异均无统计学意义(均P>0.05),但T等位基因频率的差异有统计学意义(P<0.05)。结论 AGT基因的TT型是老年高血压的危险因素,但不是糖尿病的危险因素。

Objective To investigate correlation of the angiotensinogen（AGT） polymorphism, elder- ly hypertension and elderly patients with hypertension and diabetes. Methods The analysis of polyme- rase chain reaction （PCR） and restriction fragment length polymorphism on 60 eases in the healthy elderly control group, 105 cases in the elderly hypertensive group, 38 cases in the elderly patients with hyperten- sion and diabetic group and by means of using M235T polymorphism detection. Results The frequency of MT and TI＂ genotype and T allele frequency in the group of patients with elderly hypertension were 0.371, 0. 457 and 0.643 respectively and in the group of elderly patients with hypertension and diabetes were 0. 289,0. 632 and 0. 776 respectively. Both were significantly higher than those in the healthy elder- ly control group which were 0. 167,0. 133 and 0.217 respectively （ all P 〈0.05 ）. In the group of elderly patients with hypertension and the elderly with hypertension and diabetes patients, there were no signifi- cant difference for the MT and TI？ genotype （ P 〉 0.05 ）, but there was significant difference for T allele frequency （ P 〈 0.05 ）. Conclusion The AGT gene and the TT genotype are resk factors of elderly hyper- tensive incidency, but not of diabetes.