下肢曲张静脉壁MMP-2 MMP-9和胶原含量的临床研究

Clinical study of MMP-2,MMP-9 and collagen in lower limb varicose veins

目的研究曲张大隐静脉管壁基质金属蛋白酶(MMP)-2、MMP-9的表达及其与临床症状和体征分期的关系,并检测曲张静脉壁总的胶原含量,探讨MMP-2、MMP-9和胶原在曲张静脉重塑时的作用。方法采用免疫组化SP法检测曲张静脉壁和正常大隐静脉壁MMP-2和MMP-9的表达,采用Masson染色法检测曲张静脉壁和正常对照组总的胶原含量,采用χ2检验和t检验进行统计学分析处理。结果 MMP-2和MMP-9在正常静脉壁和曲张静脉壁均有表达,但曲张静脉壁MMP-2和MMP-9的阳性率明显升高(P<0.05);C4-C6期MMP-2和MMP-9的阳性率明显高于C1-C3期(P<0.05);曲张静脉壁总的胶原含量显著高于正常对照组(P<0.05)。结论MMP-2、MMP-9的异常表达导致了静脉壁总的胶原含量增加,参与了曲张静脉的重塑。

Objective To investigate the expression of matrix metalloproteinase -2 and -9 in varicose veins, and their relationships with clinical stages , and to discuss the changes of total collagen in varicose veins, and their significance. Methods The expression of MMP-2 and MMP-9 was examined by immunohistochemistry SP methods in varicose veins （VV） and normal veins （NVV）, the total collagen was detected using Masson staining in varicose veins and normal veins, and chi-square test and T- test was used for statistical analysis. Results MMP-2 and -9 were both expressed in NVV and VV, and the positive expression rate in VV was significantly higher than that in NYV （P〈 0.05）. The positive expression of MMP-2 and -9 was significantly higher in C4-C6 stages than in C1-C3 stages （P 〈0.05）, and the collagen was significantly increased in varicose veins （P 〈0.05）. Conclusion The abnormal expression of MMP-2 and -9 leads to collagen increasing in varicose veins, which may participate in vasculkar remodeling in varicose veins.