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手足口病EV71疫苗纯化的工艺 被引量:2

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摘要 为提高肠道病毒71型(EV71)疫苗的纯化工艺,采用粗纯、4FF层析、离子交换层析、蔗糖密度梯度离心等方法进行疫苗的纯化。结果表明,粗纯的EV71浓缩液经4FF层析和蔗糖密度梯度离心,得到高纯度的EV71疫苗。新工艺提高了EV71的纯度,适用于规模化生产。
出处 《江苏农业科学》 CSCD 北大核心 2012年第6期205-206,共2页 Jiangsu Agricultural Sciences
基金 河南省政府决策研究课题(编号:B578)
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  • 1van der Sanden S,Koopmans M,Uslu G. Epidemiology of enterovirus 71 in the Netherlands,1963 to 2008[J].Journal of Clinical Microbiology,2009,(09):2826-3283.
  • 2中华人民共和国国家卫生和计划生育委员会.2013年9月全国法定传染病疫情概况.
  • 3Wu CN,Lin YC,Fann C. Protection against lethal enterovirus 71 infection in newborn mice by passive immunization with subunit VP1 vaccines and inactivated virus[J].VACCINE,2002,(5-6):895-904.
  • 4Chung YC,Ho MS,Wu JC. Immunization with viruslike particles of enterovirus 71 elicits potent immune responses and protects mice against lethal challenge[J].VACCINE,2008,(15):1855-1862.
  • 5Foo DG,Alonso S,Phoon MC. Identification of neutralizing linear epitopes from the VP1 capsid protein of enterovirus 71 using synthetic peptides[J].Virus Research,2007,(01):61-68.
  • 6Jia CS,Liu JN,Li WB. The cross-reactivity of the enterovirus 71 to human brain tissue and identification of the cross-reactivity related fragments[J].VIROLOGY JOURNAL,2010.47.
  • 7Chen HF,Chang MH,Chiang BL. Oral immunization of mice using transgenic tomato fruit expressing VP1 protein from enterovirus 71[J].VACCINE,2006,(15):2944-2951.
  • 8Arita M,Nagata N,Iwata N. An attenuated strain of enterovirus 71 belonging to genotype a showed a broad spectrum of antigenicity with attenuated neurovirulence in cynomolgus monkeys[J].Journal of Virology,2007,(17):9386-9395.
  • 9Arita M,Shimizu H,Nagata N. Temperature-sensitive mutants of enterovirus 71 show attenuation in cynomolgus monkeys[J].Journal of General Virology,2005,(05):1391-1401.
  • 10Liu L,Zhang Y,Wang J. Study of the integrated immune response induced by the inactivated EV71 vaccine[J].PLoS One,2013,(01):e54451.

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