S100A7 mRNA和蛋白在胃癌组织中的表达及其意义

Signif icance of expression of S100A7 mRNA and protein in gastric carcinoma

目的:检测S100A7mRNA和蛋白在胃癌组织中的表达情况,分析其与胃癌临床病理学参数的关系.方法:应用原位杂交技术和免疫组织化学SP法分别检测53例胃癌组织和53例正常胃黏膜组织中S100A7mRNA和蛋白的表达,并分析其表达与临床病理学参数之间的关系.结果:胃癌组织中S100A7mRNA和蛋白表达的阳性率(分别为77.36%和71.70%)显著高于正常胃黏膜组织(阳性率分别为15.09%和13.21%),χ2分别为41.330、37.110(均P=0.000).此外,S100A7mRNA和蛋白的表达与患者年龄、性别无关(均P>0.05),但与胃癌患者的分化程度、浸润深度、TNM分期和淋巴结转移密切相关(均P<0.05).结论:S100A7的高表达可能在胃癌的发生发展中发挥重要作用,其高表达可能是判定胃癌恶性程度十分重要的分子指标.

AIM：To investigate the expression of S100A7 mRNA and protein in gastric carcinoma,and to analyze the relationship between S100A7 expression and clinicopathological features of gastric carcinoma.METHODS：In situ hybridization and immunohistochemistry were used to detect the expressions of S100A7 mRNA and protein in 53 cases of gastric carcinoma and 53 cases of normal gastric mucosa,respectively.SPSS13.0 software was utilized to analyze the relationship between S100A7 expression and clinicopathological features of gastric carcinoma.RESULTS：The positive rates of S100A7 mRNA and protein expression in gastric carcinoma were significantly higher than those in normal gastric mucosa（77.36% vs 15.09%,71.70% vs 13.21%;χ2 = 41.330,37.110,both P = 0.000）.Expression of S100A7 mRNA and protein was not associated with age or sex（both P 〉 0.05）,but was closely related to differentiation degree,invasion depth,TNM stage and lymph node metastasis in gastric carcinoma（all P 〈 0.05）.CONCLUSION：S100A7 overexpression may play a pivotal role in the occurrence and development of gastric carcinoma,representing a novel marker for evaluating the malignant degree of gastric carcinoma.