sFRP-1在胰腺导管腺癌组织中的表达及其临床意义

Clinical significance of sFRP-1 protein expression in pancreatic ductal adenocarcinoma

目的:检测sFRP-1在不同胰腺组织(正常胰腺导管、胰腺导管腺癌及癌前病变胰腺上皮内瘤变和胰腺导管内乳头状粘液性肿瘤)中的表达水平,分析其在胰腺癌发生过程中的作用,为胰腺癌的临床诊疗提供新线索.方法:免疫组织化学SP法检测sFRP-1在21灶正常胰腺导管(normal pancreatic duct,NP),73灶胰腺上皮内瘤变(pancreatic intraepithelial neoplasia-1,PanIN-1),29灶PanIN-2,16灶Pan I N-3,20例胰腺导管内乳头状粘液性肿瘤(IPMN-adenoma,IPMA),13例(IPMN-borderline,IPMB),19例(IPMN-carcinoma,IPMC)及50例胰腺导管腺癌(pancreatic ductal adenocarcinoma,PDAC)中的表达,并分析sFRP-1的表达与临床病理特征及患者术后生存期的关系.结果:sFRP-1的免疫组织化学评分在由NP导管→PanIN-1→PanIN-2、PanIN-3、PDAC及NP→IPMA、IPMB→IPMC→PDAC的逐级进展过程中逐渐升高,sFRP-1的表达与肿瘤分化程度、神经浸润显著相关.结论:sFRP-1的异常表达是PDAC发生中的早期事件,sFRP-1高表达是PDAC神经浸润的重要分子特征.

AIM：To detect the expression of secreted frizzled-related protein 1（sFRP-1） protein in different pancreatic tissues（normal pancreatic duct,NP;pancreatic ductal adenocarcinoma,PDAC;pancreatic intraepithelial neoplasia,PanIN;and intraductal papillary mucinous neoplasm,IPMN） and evaluate its signif icance in the carcinogenesis of PDAC.METHODS：Immunohistochemical detection of sFRP1 protein was performed in 21 foci of NP,73 foci of PanIN-1,29 foci of PanIN-2,16 foci of PanIN-3,20 cases of IPMN-adenoma（IPMA）,13 cases of IPMN-borderline（IPMB）,19 cases of IPMN-carcinoma（IPMC）,and 50 cases of PDAC.The correlation between sFRP-1 expression and clinicopathologic characteristics of PDAC was analyzed.RESULTS：Immunohistochemical score（IHCS） of sFRP1 expression escalated with the severity of tissue atypia along the progressive multistage：NP→PanIN-1→PanIN-2,PanIN-3,PDAC or NP →IPMA,IPMB→IPMC,PDAC.sFRP-1 expression was signif icantly associated with tumor differentiation and neural inf iltration.CONCLUSION：sFRP-1 expression enhancement is an early event in the carcinogenesis of PDAC,representing an important molecular characteristic of neural inf iltration.