水蛭素活性因子对荷瘤鼠肝癌及Th1/Th2类细胞的影响

Effects of hirudin active factor on liver tumor and Th1/Th2 cells in mice with tumor

目的探讨水蛭素活性因子对肝癌实体瘤及Th1/Th2类细胞因子的作用机制,为临床用药提供理论依据。方法取小鼠48只,随机分为模型对照组、5-Fu组、水蛭素活性因子大剂量组、水蛭素活性因子小剂量组。接种H22肝癌瘤细胞,连续用药14 d,剥离肿块,比较各组肿瘤生长情况,并计算抑瘤率。取肝癌实体瘤组织标本,提取细胞总RNA进行Th1/Th2类细胞因子检测,紫外分光光度计测出光值。并进行反转录(RT)反应、聚合酶链反应(PCR)、电泳鉴定,定量分析,图像输入分析系统,进行表达强度分析。结果水蛭素活性因子对小鼠肝癌实体瘤具有明显的抑制作用,大剂量组和小剂量组抑制率分别为46%和43%。荷瘤鼠用药后,IL-2、IFN-γ细胞因子呈高表达,IL-4、IL-10细胞因子呈低表达。结论水蛭素活性因子能提高荷瘤鼠IL-2、IFN-γ细胞因子的抗癌水平,使机体由Th2类细胞向Th1类细胞逆转,增强了细胞的免疫应答能力。

Objective It is to explore the mechanism of hirudin active factor on Heps and Thl/Th2 cells, thus to provide theoretical basis for clinical pharmacy. Methods 48 mice were chosen and randomly divided into model control group, 5 - Fu group, high and low dosage of hirudin active factor groups, and they were inoculated with H22 Heps oncocyte. After continu- ously using medicine for 14 days, the tumors were peeled and their growth and calculate tumor inhibition rate were compared. Heps specimen was gotten to extract total cell RNA to determine Thl/Th2, and light value was detected by ultraviolet spectrometer. Reverse transcription （RT） reaction, polymerase chain reaction （PCR）, eleetrophoresis appraisal, quantitative analysis was performed on, and image was input to analytical system to analyze expression level. Results Hirudin active factors had obvious inhibition effect on mouse Heps. The tumor inhibition rates were 46% and 43% respectively. After using medicine, in tumor borne mouse, cell factors IL - 2, IFN - γ showed high level and cell factors IL - 4, IL - 10 showed low level. Conclusion Hirudin active factors can improve antieancer level of cell factors IL - 2, IFN - 3＇, reverse organism from Th2 cells to Thl cells and improve immune response ability of cells.