摘要
采用细菌学方法与分子生物学技术对疑似山羊痘猝死山羊进行病原学检测。结果:以GPV ITR基因特异性引物从皮肤、肠黏膜、蹄部痘疹中均扩增到289 bp目的条带,并从肝脏中分离到1株革兰氏阳性杆菌,经生化鉴定为产气荚膜梭菌,其表现较强耐药性。由试验结果初步判断,该病例为山羊痘病毒(GPV)继发产气荚膜梭菌感染所致。猝死山羊病因的确定性诊断,将对山羊养殖场切断病原传播,预防健康山羊发病提供技术支持。
The suspected goats infected with goatpox were detected by bacteriologic methods and molecular biotechnology. The results showed that it was amplified 289bp specific strap from the skin, intestinal mucosal, hoof pox with GPV ITR gene specific primers. 1 strain of the gram positive bacillus was isolated from goat liver and identified by biochemical identification as clostridium perfringcns, which had strong drug - resistance. The test result esti- mated that the case caused by goatpox virus and secondary clostridium perfringens. The diagnosis of the pathogeny of the sudden death in goats, which provided technical support to cut off spread of the pathogen on the goat＇ s farms and prevent the disease of goats.
出处
《贵州畜牧兽医》
2012年第3期14-16,共3页
Guizhou Journal of Animal Husbandry & Veterinary Medicine
关键词
山羊痘
细菌
继发感染
诊断
Goatpox
Bacterium
Secondary infection
Diagnosis.
