人工培殖冬虫夏草D-甘露醇的含量测定

Determination of D-mannitol in cultivated Cordyceps sinensis

采用水提回流离心的方法,提取分离人工培殖冬虫夏草中D-甘露醇,用高碘酸钠与甘露醇反应产生黄色的3,5-二乙酰-1,4-脱氢二甲基吡啶,在波长412 nm处测定其最大吸收。甘露醇浓度在10～50 mg/L之间时,与吸光值(OD412)有良好线性关系,线性方程为C=79.172x-0.0713,相关系数r=0.9999。人工培殖冬虫夏草中D-甘露醇的含量分别为89.64 mg/g(2005年)、91.86 mg/g(2006年)、94.71 mg/g(2007年)、98.98 mg/g(2008年)、97.50mg/g(2009年)、94.71 mg/g(野生)和96.84 mg/g(野生)。人工培殖冬虫夏草与野生冬虫夏草D-甘露醇的含量基本相同。

To provide a basis for medicinal value of cultivated Cordyceps sinensis, mannitol was separated by water extraction and centrifugation. The compound （3,5-diacetyl-1,4-dehydro-dimethylpyridine） obtained by the reaction of NaIO4 and C6 Ht2 06 had the largest absorption at wave length of 412 nm. The absorbance values（OD412 value） and mannitol concentration showed a good linearity at the range of 10 - 50 mg/L. The linear equation is C = 79. 172x - 0. 0713 （ r = 0. 9999）. The contents of mannitol in cultivated Cordyceps sinensis were 89. 64 mg/g （ 2005a, cultivated）, 91.86 mg/g （ 2006a, cultivated）, 94. 71 mg/g （ 2007a, cultivated）, 98.98 mg/g （2008a, cultivated）, 97. 50 mg/g （2009a, cultivated）, 94. 71 mg/g （2008a, wild） and 96. 84 mg/g （2008a, wild）, respectively. The results showed the mannitol content of the cultivated Cordyceps sinens＇is was equal roughly to the wild Cordyceps sinensis.