HBV感染者血清GP73的检测及临床意义

Detection and Clinical Significant of Serum GP73 in Patients Infected with HBV

目的探讨血清高尔基体蛋白73(Golgi protein 73,GP73)在不同类型乙型肝炎病毒感染者中的表达水平及临床意义。方法采用ELISA法对慢性乙型肝炎、乙肝肝硬化、HCC患者及正常对照组的血清GP73及其他血清指标进行检测,并进行Spearman相关分析;同时分析HCC患者临床及病理因素与血清GP73浓度的关系。结果不同类型乙型肝炎病毒感染者血清GP73含量均显著高于正常对照组(P<O.05)。HCC患者血清GP73含量显著其他组(P<O.05),GP73诊断HCC的ROC曲线下面积AUC达到0.865。Spearman相关分析表明HCC患者血清GP73与与ALP、AFP、LAP的相关性较高(r均>0.300,P均<0.01)。HCC患者血清GP73浓度不受HBV-DNA载量、肿瘤大小及结节数的影响。结论 HBV感染者血清GP73普遍升高,特别是HCC时升高显著,可作为一项较好的诊断HCC的血清标志物。

Objective To investigate the level of serum Golgi protein 73 （ GP73 ） and explore the clinical significant of serum GP73 in patients infected with HBV. Methods The concentration of serum GP73 in patients with chronic hepatitis B,cirrhosis, HCC and in normal controls was assayed with ELISA. Meantime,other serum markers were detected. The relationship between GP73 and other serum markers was analyzed. And the relationship between serum GP73 concentration and some clinical and pathological factors was analyzed in patients with HCC. Results GP73 level in patients infected with HBV was significantly higher than that in normal controls（ P 〈 0.05 ）. GP73 level in patients with HCC was significantly higher than that in other patients（ P 〈 0.05） and the area of ROC for HCC was 0. 865. GP73 had dose correlation with alkaline phosphatase（ALP） , alpha - fetoprotein（AFP） and leucyl aminopeptidase（LAP） （ all r 〉 0. 300, all P 〈 0.05 ）. While GP73 level was unrelated to the tumor size and number of nodules. Conclusion Serum GP73 level generally rises in patients infected with HBV,especially dramatically higher in patients with HCC. GP73 is a potential effective serum diagnosis marker for HCC.