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肿瘤相关抗原GCF2高水平表达的肝癌细胞株的筛选 被引量：7

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摘要 目的筛选出肿瘤相关抗原GCF2高表达的人肝癌细胞株,为进一步探讨GCF2对肝癌发生发展的可能作用奠定基础。方法选择4种人肝癌细胞株(BEL-7404、HepG2、SMMC-7721和QGY-7703)及1种成人正常肝细胞株(HL-7702),培养后分别制作细胞爬片及提取蛋白后,采用免疫细胞染色技术和Western blot的方法筛选出GCF2表达量高的细胞株。结果免疫细胞染色显示,GCF2在5种细胞中的胞核及胞质中均有表达。GCF2蛋白在肝癌细胞BEL-7404、HepG2、SMMC-7721和QGY-7703的表达要强于正常肝细胞HL-7702,其中以BEL-7404的表达量最高。通过Western blot检测,BEL-7404的GCF2相对表达量为0.875±0.134,较其他细胞株高,差异有统计学意义(P<0.05)。结论 BEL-7404细胞中GCF2蛋白水平的相对表达量最高,可以作为下一步探讨GCF2在肝癌发生发展的可能作用的实验材料。
作者 蒋日婷 晁耐霞 马平 李金平 罗国容
机构地区 广西医科大学组织胚胎学教研室
出处 《广东医学》 CAS CSCD 北大核心 2012年第11期1551-1553,共3页 Guangdong Medical Journal
基金 国家自然科学基金资助项目(编号:8106016) 广西科学基金资助项目(编号:2010GXNSFD013048)
关键词 肿瘤相关抗原 GCF2 肝癌 蛋白表达
分类号 R735.7 [医药卫生—肿瘤]
  • 相关文献

参考文献9

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同被引文献48

  • 1Khachigian LM, Santiago FS, Rafty LA, et al. GC factor 2 re- presses platelet-derived growth factor A-chain gene transcription and is itself induced by arterial injury [ J ]. Circ Res, 1999, 84 (11) : 1258-1267.
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  • 3Dai P, Jeong SY, Yu Y, et al. Modulation of TLR signaling by multiple MyD88-interacting partners including leucine-rich repeat Fli-I-interacting proteins [ J ] J Immunology, 2009, 182 ( 6 ) : 3450-3460.
  • 4Rikiyama T, Curtis J, Oikawa M, et al. GCF2: expression and molecular analysis of repression [ J ]. Biochim Biophys Acta, 2003, 1629(1-3): 15-25.
  • 5Stenner-Liewen F, Luo G, Sahin U, et al. Definition of tumor-as- sociated antigens in hepatocellular carcinoma [ J ]. Cancer Epide- miol Biomarkers Prey, 2000, 9 (3) : 285-290.
  • 6Ariake K, Ohtsuka H, Motoi F, et al. GCF2/LRRFIP1 promotes colorectal cancer metastasis and liver invasion through integrin-de- pendent RhoA activation [J]. Cancer Lett, 2012, 325 ( 1 ) : 99-107.
  • 7Zhang S, Liu X, Zhang Y, et al. RNAi screening identifies KAT8 as a key molecule important for cancer cell survival[ J]. Int J Clin Exp Pathol, 2013, 6(5) : 870-877.
  • 8Reed AL, Yamazaki H, Kaufman JD, et al. Molecular cloning and characterization of a transcription regulator with homology to GC- binding factor [J]. J Biol Chem, 1998, 273(34) : 21594 -21602.
  • 9Suriano AR, Sanford AN, Kim N, et al. GCF2/LRRFIP1 represses tumor necrosis factor alpha expression [J]. Mol Cell Biol, 2005, 25 (20) : 9073 -9081.
  • 10Santiago FS, Khachigian LM. Ets-1 stimulates platelet-derived growth factor A-chain gene transcription and vascular smooth muscle cell growth via cooperative interactions with Spl [ J ]. Circ Res, 2004, 95 ( 5 ) : 479 - 487.

引证文献7

二级引证文献16

广东医学
2012年 第11期

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