一株芽孢杆菌的分离鉴定及其益生潜质分析

Isolation and Identification of a Bacillus Subtilis Strain and its Potential Probiotic Analysis

从健康肉鸡的粪便中分离出一株BF3芽孢杆菌,经培养特征、形态观察、生理生化试验以及16SrDNA序列分析相似度为99%,确定该菌株为枯草芽孢杆菌,对该株芽孢杆菌对肠道致病菌的拮抗作用进行研究。结果表明BF3菌株在培养过程中,可以抑制鸡白痢沙门氏菌和鸡大肠杆菌的生长。拮抗作用的强弱与芽孢杆菌和致病菌的接种时间有关。先接种芽孢杆菌后接种致病菌,拮抗作用最强;芽孢杆菌和致病菌同时接种次之;先接种致病菌后接种芽孢杆菌,拮抗作用最弱。并且BF3菌株代谢产物对鸡白痢沙门氏菌和鸡大肠杆菌具有非常明显的拮抗作用。

A Bacillus strain BF3 ,which was isolated from the feces of healthy and adult broiler, was identified as Bacillus suhtilis by its cultural characteristics, morphological characteristics, physiological and biochemical properties and 16S rDNA sequence. Inhibition of the growth of enteric bacteria by Bacillus subtilis BF3 was studied. The results showed that the Bacillus suhtilis BF3 could decrease the propagation of the Salmonella pullorum and Chicken Escherichia coli obviously in the course of incubation. The strength of the antagonism was influenced by the inoculation time of Bacillus and pathogenic. The Bacillus strain was inoculated firstly, the antagonism was the strongest. Bacillus and pathogenic bacteria were inoculated at the same time, the antagonism ranked the second. The Enteric bacterium was inoculated firstly, the antagonism was the weakest. And the Bacillus strain BF3 metabolites showed powerful ability to inhibit the growth of the Salmonella pullorum and Chicken Escherichia coli.