单向灌流法评价栀子厚朴汤方剂配伍对栀子苷肠吸收的影响

Effect of Different Formula Compatibility of Zhi-Zi-Hou-Pu Decoction on the Intestinal Absorption of Geniposide

目的:考察大鼠在体灌流栀子、栀子-枳实、栀子-厚朴和栀子厚朴汤4种汤剂后栀子苷在大鼠肠道吸收情况。方法:建立大鼠在体单向肠灌流模型,应用高效液相色谱法测定大鼠肠灌流液中栀子苷的浓度,计算不同配伍组栀子苷吸收参数。结果:栀子-枳实、栀子-厚朴配伍以及栀子厚朴汤全方中栀子苷的吸收速率常数(Ka)和有效渗透系数(Peff)显著大于栀子单味汤剂的吸收参数(P<0.05)。结论:栀子厚朴汤方剂配伍,增加了主要药效物质栀子苷的吸收,该方剂配伍具有一定合理性。

Objective： To investigate the interaction of compatibility of Zhi-Zi-Hou-Pu decoction,including ZZ（Gardeniea fructus）,ZZ-HP（Gardeniea fructus compared with Magnoliea officinalis cortex）,ZZ-ZS（Gardeniea fructus compared with Aurantii fructus immaturus）and ZZHPD（Zhi-Zi-Hou-Pu decoction）.Methods： The rat single pass intestinal perfusion model was employed and the concentration of geniposide in intestinal samples was determined by HPLC.Results： Absorption rate constant（Ka） and effective permeability coefficient（Peff） of geniposide in the intestine significantly increased when ZZ was combined with HP or/and ZS.Conclusion： The intestinal absorption of geniposide in ZZ single extract was significantly different from those in compatibilities of ZZ-HP,ZZ-ZS and ZZHPD.Formula compatibility of Zhi-Zi-Hou-Pu decoction could promote the absorption of some major pharmacodynamic ingredients and this traditional Chinese medicine compound had some rationality.