摘要
目的检测系统性红斑狼疮(SLE)患者CD200/CD200R1的表达,探讨CD200/CD200R1信号对Th17/Treg平衡的影响。方法用流式细胞仪检测53例SLE患者及24名健康对照者CD4+T细胞以及树突细胞表面CD200和CD200R1的表达,用酶联免疫吸附试验检测受试者血清游离CD200水平。通过体外细胞刺激培养观察CD200/CD200R1信号对Th17分化及调节性T细胞Treg生成的影响。结果 SLE患者CD4+T细胞、树突细胞CD200的表达及血清游离CD200水平均显著高于健康对照者,差异有统计学意义(P<0.05);CD200R1显著低于健康对照者,差异亦有统计学意义(P<0.05)。体外细胞培养给予CD200-Fc融合蛋白可降低SLE患者Th17的分化并纠正TGF-β诱导的调节T细胞生成缺陷。结论 SLE患者CD200和CD200R1表达异常,CD200/CD200R1信号在Th17/Treg平衡中具有重要作用。
Objective CD200 is a type I transmembrane glycoprotein that can polarize cytokine production and maintain immune homeostasis.We detected the expression of CD200/CD200R1 and evaluate the effect of CD200/CD200R signaling on the balance of Treg/Th17 in patients with systemic lupus erythematosus（SLE）.Methods Serum CD200 level was detected by ELISA,and the expression of CD200 and CD200R1 by CD4＋ T cells and dendritic cells（DCs） from 53 SLE patients and 24 healthy controls（HCs） were examined by flow cytometry,and the results were compared between SLE patients and HCs.The effect of CD200 on Th17 differentiation and Treg generation was determined by cell culture assay.Results In SLE patients,the number of CD200＋ cells and the level of soluble CD200 were significantly higher than in HCs（P〈0.05）,whereas the expression of CD200R1 by CD4＋T cells and DCs was decreased（P〈0.05）.The engagement of CD200 receptor on CD4＋T cells with CD200-Fc fusion protein in vitro reduced the differentiation of Th17 cells and reversed the defective induction of CD4＋CD25highFoxP3＋T cells by TGF-β in SLE patients.Conclusion CD200 and CD200R1 expression is abnormal in SLE and may contribute to the balance of Treg/Th17 in SLE.
出处
《中华临床免疫和变态反应杂志》
2012年第2期92-97,共6页
Chinese Journal of Allergy & Clinical Immunology
基金
国家自然科学基金(81172859)
