溶藻菌发酵液及其溶藻产物的生物急性毒性试验

Acute toxicity of the fermentation broth of algae-lying bacteria and its products

应用发光细菌(Photobacterium phosphoreum)急性毒性试验,研究了溶藻菌(Streptomyces sp.HJC-D1)发酵液及其对铜绿微囊藻(Microcystis aeruginosa)抑制产物的生物急性毒性。结果表明,溶藻菌发酵液本身对发光细菌具有一定的低毒性,发酵3～5 d时其相对发光度为(67.59%±3.11%)～(72.35%±2.76%);体积分数5%的溶藻菌发酵液可有效抑制初始质量浓度高达(0.148 3±0.003 2)mg·L-1的铜绿微囊藻生长,其抑藻率达85%以上,且藻液毒性明显低于对照组;以微囊藻毒素为主要溶藻产物进行毒性试验发现,其半抑制质量浓度为1 096.92μg·L-1,水体藻毒素质量浓度低于20μg·L-1时其生物毒性较低。

The acute toxicity of fermentation broth of algae-lying bacteria Streptomyces sp. HJC-D1 and its products treating Microcystis aeruginosa was studied with Photobacterium phosphoreum. The results indicated that the fermentation broth of algae-lying bacteria had certain biotoxicity via photobacteria tests, and the relative luminosity was at （67.59%±3.11%）-（72.35%±2.76%） after 3-5 days＇ fermentation. The inhibitory efficiency of M. aeruginosa was above 85% at the initial concentration of （0.1483±0.0032） mg.Ll by adding fermentation broth （5%, V/V）, and it was obviously that the acute toxicity of the test groups was lower than the control groups. The toxicity tests with Microcystin-LR as the main algae-lying product showed that, the EC50 value （half inhibition concentration for 15 min） of Microcystin-LR is 1096.92 μg.L1 and there＇s no inhibited effect when the concentration of Microcystin-LR is lower than 20 μg.L-l.