摘要
目的:建立衍宗育子胶囊中淫羊藿苷的含量测定方法。方法:采用高效液相色谱法,Appollo C18柱(4.6 mm×250 mm,5μm),乙腈-水(26∶74)为流动相,检测波长270 nm,柱温室温。结果:淫羊藿苷在0.025 5～0.408μg,峰面积与其质量浓度呈良好线性关系(r=0.999 8),平均回收率为99.4%,RSD 1.25%(n=6)。结论:该方法简便、准确、重复性好,可作为该制剂含量测定方法。
Objective： To develop an HPLC method for detecting icariin in Yanzong Yuzi capsules Method： An Appollo C18column （4.6 mm × 250 mm, 5 μm） was used. The mobile phase consisted of acetonitrile-water （26： 74）. The detection wave length was set at 270 nm and the column temperature was at room temperature. Result： Icariin showed a good linear relationship in the range of 0. 025 5 to 0. 408 p.g, r = 0. 999 8. The recovery and RSD were 99.4% and 1.25% （n =6）. Conclusion： Method was proved to be simple, accurate and repeatable to determine the content of icariin in Yanzong Yuzi capsules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第13期65-66,共2页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西区卫生厅自筹经费科研课题(Z2011521)
