星毛冠盖藤的质量研究

The Study on Quality of Pileostegia tomentella

目的:检查所采星毛冠盖藤是否符合药典要求,进一步开发其资源。方法:采用2010年版《中国药典》方法对星毛冠盖藤进行了水分、灰分及浸出物含量检测,考察提取方法及提取时间对浸出物含量的影响;以70%乙醇回流提取总黄酮,芦丁为对照品采用紫外分光光度法进行含量测定。结果:水分含量为10.8%,总灰分为5.2%,酸不溶灰分为1.2%,水溶性浸出物含量以回流方法提取40 min最高,提取率达13.45%,醇溶性浸出物以回流方法提取60 min最高,提取率为11.09%;总黄酮含量为0.39%,回收率102.7%,RSD 1.76%,其他方法学考察均符合要求。结论:所采星毛冠盖藤样品符合要求。为该植物资源的合理利用、质量控制及进一步开发提供了理论基础。

Objective： To detect if Pileostegia tomentella collected is qualified according to Chinese Pharmacopoeia for advanced study. Method： Use the method of Chinese Pharmacopoeia （2010 edition） to detect the content of water, ash and extract from P. tomentella, investigate the influence of different extract methods and time for extract content. Flavones were extracted with 70% ethanol by reflux and determinated by ultraviolet spectrophotometry, which rutin was taken as control sample. Result： The water content, total ash content and the acid-insoluble ash content was 10.8% , 5.2% and 1.2% , respectively. The content of highest water soluble extract is 13.45% by reflux extraction for 40 mins, the highest ethanol-soluble extractives is 11.09% by reflux extraction for 60 mins, the total flavones content is 0.39% with good repeatability. The recovery rate was 102.7% and RSD was 1.76%. Conclusion： The research provide basis of theoretical for the reasonable use, quality control, development and industrialization production.