补肾活血方对肾阳虚大鼠耳蜗组织Fas mRNA表达的影响

Influence of Bushen Huoxue Fang on the Expression of Cochlea Fas mRNA in the Kidney-deficiency Rats

目的:观察补肾活血方对肾阳虚后大鼠耳蜗组织Fas mRNA表达的影响。方法:将耳廓反应灵敏的健康SD大鼠随机选取8只用于空白对照组,其余16只用于肾阳虚造模。采用氢化可的松注射液0.02 g·kg-1im的方法造成肾阳虚模型,造模14 d后,分为模型组、补肾活血方ig 3.1 g·kg-1组。治疗组造模结束后开始给药,连续ig给药2周。给药结束后测试大鼠听性脑干反应(ABR),随后处死动物。股动脉取血测试环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP);在超净工作台上剥离耳蜗,用RT-PCR的方法检测耳蜗组织Fas mRNA的表达。结果:空白组耳蜗Fas mRNA的表达为0.210 1±0.025 6,模型组Fas mRNA的表达为0.551 4±0.090 3,补肾活血方组Fas mRNA的表达0.242 9±0.052 5。经统计学分析,模型组大鼠耳蜗组织Fas mRNA的表达与空白组相比,明显上调,具有统计学意义(P<0.01);补肾活血方可降低肾阳虚大鼠耳蜗Fas mRNA的表达,与模型组比较,差异有统计学意义(P<0.01)。结论:耳蜗组织中Fas蛋白的改变可能是促进肾阳虚后耳蜗病变发展的因子之一,补肾活血方可能通过调节Fas mRNA的表达而起到提高听力,改善耳蜗结构的作用。

Objective： To observe the effect of Bushen Huoxue Fang on the expression of cochlea Fas mRNA in the kidney-deficiency rats. Method： Eight healthy SD rats with a sound auricle response were randomized into the control group, the other 16 rats were modeled into kidney-yang-deficiency by alternate injection of cetacort into their buttocks. After 14 days of modeling, rats were randomized into model group, Bushen Huoxue Fang group. The treatment lasted two weeksn. Rats in each group were chosen randomly to test auditory brain-stem response （ABR） on the day 15 of treatment, then all rats were sacrificed. The blood of the femoral artery was collected to measure the cyclic adenosine monophosphate （cAMP） and cyclic guanosine monophosphate （cGMP）. Cochlea was isolated, and RT-PCR method was adopted to test the expressions of Fas mRNA. Result： The Fas mRNA expression in control group was 0. 210 1 ± 0. 025 6, and 0. 551 4 ± 0. 090 3 in model group, but Bushen Huoxue Fang was 0. 242 9 ± 0. 052 5. The model group enhanced the Fas mRNA expression compared with the control group （P 〈 0.01 ） ; Bushen Huoxue Fang decreased the Fas mRNA expression, compared with model group （P 〈 0.01 ）. Conclusion： Bushen Huoxue Fang may regulates the mRNA expression of Fas, therefore can improve the hearing and protect the structure and function of cochlea.