香蕉皮多酚对高脂血症大鼠降血脂作用的实验研究

Study on the Antilipemic Effect of Banana Peel Polyphenols in Hyperlipidemic Rats

目的:研究香蕉皮多酚对高脂血症模型大鼠的降血脂作用。方法:健康SD雄性大鼠60只随机分为6组:正常对照组(NC)、模型对照组(MC)、阳性对照组(PC,辛伐他汀组)、香蕉皮多酚低剂量组(P1)、香蕉皮多酚中剂量组(P2)、香蕉皮多酚高剂量组(P3)。NC组给予基础饲料;MC组给予高脂饲料;PC组给予高脂饲料,并灌胃给予辛伐他汀10 mg·kg-1,1次/日;P1,P2,P3组给予高脂饲料,并分别灌胃给予香蕉皮多酚(30,60,120 mg·kg-1),1次/日;均自由饮水,每周称1次大鼠体重。各组按规定给药40 d后,禁食12 h,眼眶静脉取血,分离血清,测定血清总胆固醇(TC)、血清三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)。结果:大鼠用高脂饲料喂养40 d后,与正常对照组相比,模型对照组TG(1.14±0.21)mmol·L-1,TC(3.06±0.24)mmol·L-1,LDL-C(2.97±0.12)mmol·L-1,水平明显升高,HDL-C(0.89±0.11)mmol·L-1,水平降低(P<0.05);治疗组给予香蕉皮多酚后,与模型对照组相比,TC,TG和LDL-C水平明显降低,HDL-C水平升高(P<0.05)。结论:香蕉皮多酚具有一定的降血脂预防动脉粥样硬化形成的作用。

Objective： To study the antilipemic effect of banana peel polyphenols in hyperlipidemic rats. Method： Sixty healthy male SD rats were randomly divided into six groups： normal control group （NC） , model control group （MC）, positive control group （PC, simvastatin group）, low dose group of banana peel polyphenols （PI） , middle dose group of banana peel polyphenols （P2） , high dose group of banana peel polyphenols （ P3 ）. The rats in the normal control group were fed with normal diet, and the MC groups were given high fat diet; the PC groups were given high fat diet, and given orally administrated with simvastatin at dose of 10 mg. kg^-1, semel in die; the P1, P2, P3, groups were given high fat diet, and orally administrated with banana peel polyphenol （30, 60, 120 mg.kg^-1）, semel in die. After 40 days rats were sacrificed and blood sample was collected to evaluate serum total cholesterol （TC） , serum triglyceride （TG） , low-density lipoprotein cholesterol （LDL-C） , high density lipoprotein cholesterol （HDL-C）. Result： Compared with the NC group, the level of TG （1.14 ± 0.21 ） mmol. L^-1, TC （3.06±0.24） mmol. L^-1, LDL-C （2.97±0.12） mmol. L^-1 in MC group was increased for, and HDL-C （0. 89 ± 0. 11 ） mmol. L^-1 level was decreased in rats fed high fat diet （P 〈 0.05） , and then the levels of TC, TG and LDL-C in the treatment groups were decreased, and HDL-C levels were increased （P 〈 0.05）.Conclusion： It demonstrates that the banana peel polyphenols could effectivly reduce the levels of blood lipid in hyperlipidemic rats.