白背叶提取物芹菜素对裸鼠人胃癌细胞移植瘤生长及凋亡的影响

Effect of Apigenin from Mallotus apelta on Proliferation and Apoptosis in Nude Mice Bearing Human Gastric Carcinoma

目的:研究白背叶提取物芹菜素对裸鼠人胃癌SGC-7901细胞移植瘤生长及凋亡的影响。方法:建立人胃癌裸鼠移植瘤模型,连续11 d ig给予芹菜素600,300,150 mg·kg-1或ip五氟尿嘧啶(5-FU)20 mg·kg-1治疗,观察移植瘤瘤重及药物对肿瘤生长的抑制率;透射电镜观察肿瘤组织细胞凋亡情况。结果:芹菜素600,300 mg·kg-1和5-FU均能明显抑制裸鼠移植瘤生长,抑瘤率分别为31.78%(P<0.01),25.66%(P<0.05)和49.03%(P<0.01),且芹菜素可致裸鼠移植瘤大量细胞发生凋亡,可见典型的细胞凋亡形态学变化。结论:白背叶提取物芹菜素可能通过抑制肿瘤细胞增殖、诱导细胞凋亡而发挥抗肿瘤作用。

Objective： To investigate the cytotoxic effects of apigenin from MaUotus apelta, on proliferation and apoptosis in nude mice bearing human gastric carcinoma. Method： The model of nude mice bearing developed xenografts of the SGC-7901 cell line was established, and mice were administered with apigenin at dose of 600, 300, 150 mg. kg^-1 by oral and 5-FU at dose of 20 mg .kg^-1 by intraperitoneal injection （ip） respectively for 11 days. The weights of the tumor and inhibitory rate of tumor of the nude mice were observed. The morphological changes of apoptosis were observed by transillumination electron microscope. Result： In tumor bearing mice, apigenin at dose of 600, 300 mg. kg^-1 and 5-FU could clearly prevented tumor growth in nude mice. The inhibitory rates were 31.78% （P〈0.01） and 25.66% （P〈0.05） and 49.03% （P〈0.01）. The nude mice treated with apigenin showed significant morphological changes in different apoptotic phases. Conclusion： The apigenin from M. apelta has obvious antitumor effects and the mechanism may be related to inhibited proliferation and induction of apoptosis in cancer cells.