不同滋阴中药对小鼠诱发性肺肿瘤发生及抗肿瘤免疫功能的影响

Effect of Different Traditional Chinese Medicine for Nourishing Yin on Lung Chemical Carcinogenesis and Antitumor Immune Function in Mice

目的:研究六味地黄丸和二冬膏对乌拉坦诱发性肺肿瘤小鼠的肿瘤发生情况和抗肿瘤免疫功能的影响。方法:雌性昆明种小鼠33只,使用乌拉坦(1 g·kg-1·d-1)注射诱发小鼠肺肿瘤,随机分为对照组、六味地黄丸组(ig 1.56 g·kg-1·d-1)和二冬膏组(ig 0.78 g·kg-1·d-1),连续给药100 d后肉眼及病理切片和苏木精-伊红染色观察药物对小鼠肺肿瘤发生的影响,测定脾指数、胸腺指数、巨噬细胞吞噬功能以及测定血清中肿瘤坏死因子-α(TNF-α)含量。结果:与对照组比较,六味地黄丸和二冬膏给药组小鼠肺肿瘤诱发率明显降低,诱发率分别是63.6%,18.2%,36.4%。与对照组比较,六味地黄丸和二冬膏给药组小鼠脾指数、巨噬细胞活性明显增高(P<0.05或P<0.01),小鼠血清中TNF-α含量明显降低(P<0.05或P<0.01)。结论:六味地黄丸和二冬膏均能通过提高小鼠的抗肿瘤免疫功能和减少炎症因子产生来延缓乌拉坦诱发肺肿瘤的发生发展。

Objective： The effect of Liuwei Dihuang Wan （LWDH） and Erdong Gao （ED） on the tumorigenesis and the antitumor immune function were studied in a model of chemical carcinogenesis in the mouse lung. Method： Thirty three female Kunming mice with urethane subcutaneous injection （ 1 g. kg^-1 . d^-1 ） were randomized into three groups： the control group, the group treated with LWDH （ig, 1.56 g.kg^-1.d^-1） and the group treated with ED （ig, 0.78 g. kg^-1 .d^-1）. The mice were sacrificed after 100 days to investigate the rate of tumor formation by naked eye, pathological section and hematoxylin-eosin staining. The spleen index, thymusindex, activity of macrophage and content of tumor necrosis factor alpha （TNF-α） in the blood serum were tested. Result： In the process of lung carcinogenesis, compared with control group, LWDH and ED could reduce the rate of lung chemical carcinogenesis which was 18.2% and 36.4%, respectively. Compared with control group, LWDH and ED could also increase the spleen index, elevate the activity of macrophage （P 〈 0.05 or P 〈 0. 01 ） and decrease the content of TNF-α in the blood serum （P 〈0.05 or P 〈0.01 ）. Conclusion： LWDH and ED can delay the occurrence and development of lung carcinoma induced by urethane through enhancing antitumor immunity and decreasing content of the inflammatory factor.