舒肝胶囊对“怒”伤大鼠的行为及生殖器官影响的研究

Effects of Shugan Capsule on Behavior and Generative Organ in Rats with Anger Stress

目的:研究观察中药舒肝胶囊对长期"怒"心理应激雌性大鼠的行为学和体重、子宫及卵巢指数的改变。方法:将24只雌性SD大鼠随机分为3组(正常对照组、"怒"模型组、舒肝胶囊组),每组8只。模型组予夹尾间接激怒法造模4周,舒肝胶囊组在造模的同时予舒肝胶囊0.54 g·kg-1灌胃,给药4周。另外群居饲养10只大鼠作为入侵者激怒。记录并分析各实验组大鼠的行为学改变,体重及子宫、卵巢的指数,评价模型质量及中药干预的效果。结果:模型组较正常组水平、垂直及总得分均显著增高(P<0.01),中央格时间、粪便粒数增多,修饰次数、修饰时间显著减少(P<0.01);体重减轻(P<0.05);卵巢指数显著降低(P<0.01);舒肝胶囊组较模型组水平、垂直及总得分均显著降低,差异有显著性(P<0.01);中央格时间延长、粪便粒数减低,修饰次数及修饰时间增多(P<0.05或P<0.01);卵巢指数显著增高(P<0.01)。结论:舒肝胶囊对改善"怒"引起的敏感、紧张情绪有一定的改善作用,对缓解"怒"引起的体重、子宫及卵巢指数的改变有一定效果。

Objective： To investigate the effects of Shugan capsule （SGC） on behavior, body weight, uterus and ovary index in rats with psychological stress of angero Method： Animal model of anger stress was established by indirectly clamping the mice tails according to ZHAN Xianghong＇s method. The SGC group was given SGC 0.54 g.kg^-1.d ig for 4 weeks. The behavior was recorded and analyzed as well as BW, uterus and ovary index were used to evaluate the model and effects of SGC. Result： Theopen field test showed that each index changed significantly in model group compared with normal control group （P 〈 0.01 ）, but SGC group have no significant discrepancy; compared with model group, SGC group have significant discrepancy （P 〈 0.05）. Body weight and organ index in model group have significant discrepancy compared with normal control group （P 〈 0. 05 or P 〈 0.01）, but SGC group have no significant discrepancy; compared with model group, SGC group have or no significant discrepancy. Conclusion： SGC can regulate ovary index. the abnormal emotion and improve BW and uterus and