过氧化氢对小鼠口腔癌发病影响的实验研究

Effect of hydrogen peroxide on 4NQO induced oral carcinogenesis

目的检测氧化应激相关基因Nrf2、GST-π、HO1在4NQO小鼠舌癌标本中的表达水平,分析氧化损伤对4NQO诱导的小鼠口腔癌的促进作用。方法 190只8周龄雄性C57BL/6J小鼠随机分为阴性对照组、阳性对照组、6%、15%和30%过氧化氢处理组。阴性对照组不做任何处理,其余4组以0.005%4NQO诱癌16周后,分别用水(阳性对照组)和6%、15%、30%的过氧化氢涂抹小鼠舌部,每周3次,共8周。第24周末处死全部动物,取舌组织进行组织病理学分析及免疫组化染色。结果在粘膜从正常到异常增生、再到癌变的过程中,Nrf2、GST-π和HO1的表达持续升高,并且30%过氧化氢组中重度异常增生和癌变的组织中上述三者的积分光密度值显著高于阳性对照组(P<0.01);6%和15%组中,GST-π的积分光密度值均显著高于阳性对照组(P<0.05),Nrf2和HO1的积分光密度值与阳性对照组无统计学差异。结论过氧化氢造成的氧化损伤进一步促进了4NQO小鼠口腔癌的发展。

Objective To examine the expression of Nrf2, GST-Ir, HO-1 in 4NQO-induced oral cancer of mouse and the effect on the carcinogenesis of 4NQO-induced oral cancer. Methods A total of 190 male C57BL/6J mice （ 8 weeks old） were divided into five groups, Group A and B serving as negative and positive control, Group C, D and E treated with hydrogen peroxide. The negative control,Group C, D and E were treated with 0. 005% 4NQO for 16 weeks,and then the tongue of these 4 groups were topically smeared with hydrogen peroxid 3 times a week for 8 weeks. At the end of 24 weeks, all the animals were sacrificed. The tongue was harvested and examined for the presence of macroscopic alterations and for histopathology. Results During the progression of oral carcinogenesis, Nri2, GST-xr and HO1 was over expressed from the premalignant to malignant stage. Conclusion Oxidative damage caused by hydrogen peroxide further promoted the carcinogenesis of 4NQO-induced oral cancer.