芘荧光探针应用于狭鳕鱼皮凝胶形成过程的可行性研究

Study on the feasibility of pyrene fluorescence probe applied in the formation of gel from walleye pollok(Theragra Chalcogramma) skin

在狭鳕鱼皮弹性水凝胶的动态形成过程中引入了芘荧光探针。结果表明,在6.67%的鱼皮明胶溶液中,芘单体的荧光强度和I1/I3值均随芘浓度的增大先升高后降低,当芘浓度为40μmol/L时,单体荧光强度达到最大值,而当芘浓度为20～60μmol/L时,I1/I3值均处于较高水平,数值相近;探针的IE/IM值随芘浓度的增大一直升高,当芘浓度为2～40μmol/L时,比值偏低。综合考虑上述三个因素,选用浓度为40μmol/L的芘作为荧光探针应用于狭鳕鱼皮水凝胶的动态形成过程。实验确定芘I1/I3值能够用来表征溶胶中分子间的交联情况,其凝胶时间点为7h;但是IE/IM值则无法表征分子间的交联。这为表征其它鱼皮凝胶的动态形成过程提供了重要基础。

The pyrene fluorescence probe was introduced in the formation of elastic gel from walleye pollock （Theragra Chalcogramma） skin. Results showed that the fluorescence intensity and 11/13 ratio of pyrene monomer both increased at first and then decreased in the sol of 6.67% with the increasing of pyrene concentration. The monomeric fluorescence intensity showed its maximum,when pyrene concentration reached 401μmol/L,while the 11/13 ratio had a high level in the range of 20N60μmol/L,and the values were very close. Pyrene Ie/IM ratio increased with the concentration increasing,and was lower in the range of 2-40μmol/L. Considering the three factors mentioned above,pyrene with the concentration of 40μmol/L was employed as the fluorescence probe in the gel formation from walleye pollock skin. The study indicated that pyrene I1/13 ratio could characterize the intermolecular cross-linking in the sol,and the gelatin point was 7h,but IdIM ratio couldn＇t. This 13rovided the important basis to characterize the dynamic formation of other fish skin aels.