响应曲面法优化乳杆菌产细菌素的条件研究

Optimization of the fermentative conditions for bacteriocin production of Lactobacillus plantarum by response surface methodology

以分离自泡菜可产细菌素的乳杆菌作为实验菌,优化其产细菌素的最佳培养条件,以提高其产细菌素的能力。通过Plackett-Burman实验筛选出对乳杆菌产细菌素有显著影响的3个因素,分别为装液量、葡萄糖质量浓度以及蛋白胨质量浓度。以抑菌圈直径大小作为产细菌素能力大小的判断依据,通过最陡爬坡实验和Box-Behnken实验进一步优化,并对优化的结果进行验证,验证结果表明,预测值和实际值有良好的拟合性,此优化模型可靠。最后确定的乳杆菌产细菌素的优化培养基组成为:蛋白胨30g/L、葡萄糖15g/L、柠檬酸氢二铵2g/L、K2HPO42g/L、乙酸钠5g/L、MnSO4.4H2O0.25g/L、MgSO4.7H2O0.58g/L、吐温800.1%;最佳培养条件为:装液量25mL、温度30℃、培养时间24h、接种量1%、pH6.0。在此优化发酵条件下,细菌素的产量提高了30%。

As one strain was isolated from traditional fermented pickle,Lactobacillus plantarum was investigated for its optimal condition of bacteriocin production. The mainly factors included the liquid volume,glucose concentration,and peptone concentration which were screened by Plackett-Burman design. The capability of bacteriocin produced was judged in accordance with size of inhibition zone diameter. With the help of Box- Behnken design,the maximum response value could be identified. The results showed good fitting between the estimated value and the real value through verification,and the model was reliable. The optimization of cultured medium and fermentation conditions of Lactobacillus plantarum was as follows .. peptone 30g/L,glucose 15g/L, diammonium hydrogen citrate 2g/L,K2HPO4 2g/L,NaAc 5g/L,MnSO4 -4H20 0.25g/L,MgSO4.7H20 0.58g/L, tween80 0.1% ,fluid volume 25mL,temperature 30℃ ,the fermentation period 24h,inoculation amount 1% and pH6.0. Under the fermentative conditions,bacteriocin production of Lactobacillus plantarum increased by 30%.